Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Centre for Healthy Eating & Food Innovation, Faculty of Science and Engineering, Maastricht University-campus Venlo, 5928 SZ Venlo, Netherlands.
Poult Sci. 2022 Nov;101(11):102113. doi: 10.1016/j.psj.2022.102113. Epub 2022 Aug 5.
This study's objective was to investigate the effects of dietary Se (in the form of selenomethionine) on the antioxidant activity and selenoprotein gene expressions in layer breeder roosters. One hundred and eighty, 36-wk-old Jingfen layer breeder roosters were randomly allocated to one of 5 dietary treatments (0, 0.25, 0.5, 1, or 2 mg/kg Se) for 6 wk on a corn-soybean meal-based diet. Antioxidant parameters and selenoprotein gene expressions were assessed at the end of the experiment. The results showed that Se supplementation significantly increased the activity of T-SOD, CAT, GSH-Px, and superoxide anion scavenging ability in plasma (P ≤ 0.05), and activities of T-SOD, CAT, GSH-Px, superoxide anion scavenging ability, and hydroxyl radical scavenging ability in the liver, kidney, and testis (P < 0.05). Moreover, MDA levels were significantly reduced in plasma, liver, kidney, and testis (P < 0.01), compared to the control group. Furthermore, the dietary administration of Se significantly increased TrxR2 and GPx4 mRNA levels in kidney and testis, and ID1 mRNA levels in liver and kidney. Most of the antioxidant parameters and selenoprotein-related gene expressions significantly increased, and MDA significantly decreased at dietary supplementation with 0.5 mg/kg Se. Whereas a higher dose of Se level (1 or 2 mg/kg) inhibited the activities of some of the antioxidant enzymes and selenoprotein-related gene expressions in selected tissues. In conclusion, dietary Se supplementation with 0.5 mg/kg significantly improved roosters' antioxidant status and selenoprotein-related gene expression in liver, kidney, and testis, while higher doses led to inhibit these; dietary Se might increase reproductive performance by enhancing their antioxidant status in roosters.
本研究旨在探讨饲粮硒（以硒代蛋氨酸形式）对蛋种鸡公鸡抗氧化活性和硒蛋白基因表达的影响。将 180 只 36 周龄京粉蛋种鸡公鸡随机分为 5 个饲粮处理组（0、0.25、0.5、1 或 2 mg/kg 硒），每个处理组 6 个重复，每个重复 6 只鸡。在玉米-豆粕基础饲粮上进行 6 周试验。试验结束时测定抗氧化参数和硒蛋白基因表达。结果表明：饲粮添加硒显著提高了血浆 T-SOD、CAT、GSH-Px 活性和超氧阴离子清除能力（P≤0.05），以及肝脏、肾脏和睾丸 T-SOD、CAT、GSH-Px 活性、超氧阴离子清除能力和羟自由基清除能力（P＜0.05）。与对照组相比，饲粮添加硒还显著降低了血浆、肝脏、肾脏和睾丸 MDA 含量（P＜0.01）。此外，饲粮添加硒还显著提高了肾脏和睾丸 TrxR2 和 GPx4 mRNA 水平以及肝脏和肾脏 ID1 mRNA 水平。饲粮添加 0.5 mg/kg 硒时，大多数抗氧化参数和硒蛋白相关基因表达显著增加，MDA 含量显著降低。而饲粮添加更高水平的硒（1 或 2 mg/kg）则抑制了一些组织中抗氧化酶和硒蛋白相关基因的表达。综上所述，饲粮添加 0.5 mg/kg 硒显著改善了公鸡的抗氧化状态和肝脏、肾脏和睾丸的硒蛋白相关基因表达，而添加更高水平的硒则抑制了这些变化；饲粮添加硒可能通过增强公鸡的抗氧化状态来提高其繁殖性能。
