Knight M, Cook J, Wispe J, Roberts R J
Dev Pharmacol Ther. 1987;10(4):245-64. doi: 10.1159/000457752.
The antioxidant properties of alpha-tocopherol and alpha-tocopheryl acetate in lung tissue were quantitated in vitro by measurement of thiobarbituric acid (TBA) reactants, pentane production and lipid peroxides following either an iron/ascorbate or a xanthine/xanthine oxidase oxidant stress. Lung homogenates were obtained from newborn rabbits treated intravenously with either 10 or 100 mg/kg of either alpha-tocopherol or alpha-tocopheryl acetate. The animals were killed 5 min after dosing to minimize the conversion of alpha-tocopheryl acetate to alpha-tocopherol. Lung homogenates from animals treated with alpha-tocopherol had decreased concentrations of TBA reactants and pentane production after incubation with either oxidant stress compared to lung homogenate from animals treated with alpha-tocopheryl acetate or vehicle alone. Lipid peroxide concentrations were no different in homogenates from alpha-tocopherol or alpha-tocopheryl acetate treated animals. Correlations between antioxidant activity and tissue concentrations of alpha-tocopherol indicate 50% inhibition is achieved with 30-100 micrograms/g lung tissue. In vitro addition of alpha-tocopherol required concentrations between 100 and 200 micrograms/g to reduce lipid peroxidation by 50%.
通过测量硫代巴比妥酸（TBA）反应物、戊烷生成量和脂质过氧化物，在铁/抗坏血酸或黄嘌呤/黄嘌呤氧化酶氧化应激后，体外定量测定肺组织中α-生育酚和α-生育酚乙酸酯的抗氧化特性。肺匀浆取自静脉注射10或100mg/kgα-生育酚或α-生育酚乙酸酯的新生兔。给药5分钟后处死动物，以尽量减少α-生育酚乙酸酯向α-生育酚的转化。与单独用α-生育酚乙酸酯或赋形剂处理的动物的肺匀浆相比，用α-生育酚处理的动物的肺匀浆在与任一氧化应激孵育后，TBA反应物浓度和戊烷生成量均降低。α-生育酚或α-生育酚乙酸酯处理的动物的匀浆中脂质过氧化物浓度无差异。抗氧化活性与肺组织中α-生育酚浓度之间的相关性表明，肺组织中α-生育酚浓度为30 - 100微克/克时可实现50%的抑制。体外添加α-生育酚需要100至200微克/克的浓度才能将脂质过氧化降低50%。
