Chen Gangfu, Wu Min, Li Huatao, Xu Jing, Liu Haijing, Du Wenhao, Yang Qihui, Feng Lin, Jiang Jun
Key Laboratory of Sichuan Province for Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River, College of Life Sciences, Neijiang Normal University, Neijiang 641100, China.
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.
Animals (Basel). 2023 Aug 4;13(15):2522. doi: 10.3390/ani13152522.
The objective of this study was to investigate the effects of extract (SDE) on stress induced by high stocking density and Cu and trichlorfon exposure in crucian carp (). The results showed that these stressors exerted detrimental effects in fish, such as inhibition of growth performance, reduced feed intake, and interruption of fish locomotion. Under high stocking density, dietary SDE supplementation increased the content of reduced glutathione (GSH) and the activities of amylase, catalase (CAT), and glutathione reductase (GR) and decreased the content of malonaldehyde (MDA) in the intestine of crucian carp. A similar trend was presented in the hepatopancreas under Cu exposure. Dietary SDE supplementation enhanced the activities of CAT, superoxide dismutase (SOD), glutathione peroxidase (GPx), lactate dehydrogenase, glutamate-oxaloacetate transaminase, and glutamate-pyruvate transaminase in the muscle of crucian carp under trichlorfon exposure. The optimum dietary SDE supplementation levels were 4.07, 4.33, and 3.95 g kg diet based on the recovery rate of weight gain (RWG), feed intake (FI), and inhibitory rate of rollover (IR) for crucian carp under high stocking density and Cu and trichlorfon exposure, respectively. Overall, dietary supplementation with SDE may be a useful nutritional strategy for relieving these stresses in aquatic animals.
本研究的目的是探讨提取物（SDE）对鲫鱼在高放养密度以及铜和敌百虫暴露情况下所诱导应激的影响。结果表明，这些应激源对鱼类产生了有害影响，如抑制生长性能、减少采食量以及干扰鱼类运动。在高放养密度条件下，日粮中添加SDE可提高鲫鱼肠道中还原型谷胱甘肽（GSH）的含量以及淀粉酶、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）的活性，并降低丙二醛（MDA）的含量。在铜暴露情况下，肝胰腺也呈现出类似趋势。在敌百虫暴露情况下，日粮中添加SDE可提高鲫鱼肌肉中CAT、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、乳酸脱氢酶、谷草转氨酶和谷丙转氨酶的活性。基于高放养密度以及铜和敌百虫暴露情况下鲫鱼的体重增加恢复率（RWG）、采食量（FI）和翻转抑制率（IR），日粮中SDE的最佳添加水平分别为4.07、4.33和3.95 g/kg日粮。总体而言，日粮中添加SDE可能是缓解水生动物这些应激的一种有效营养策略。
