College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China.
Department of Central Laboratory, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, Shandong 266035, China.
Carbohydr Polym. 2022 Nov 15;296:119975. doi: 10.1016/j.carbpol.2022.119975. Epub 2022 Aug 13.
The temperature-sensitive hydrogel (H/D) composed of hydroxybutyl chitosan (HBC) and diatom biosilica (DB) was prepared by physically mixing HBC solution with DB at low temperature. The rheological measurements confirmed that the incorporation of DB to hydroxybutyl chitosan could significantly enhance the mechanical strength of the hydrogels without disrupting its reversible phase transition behavior. The hierarchical porous structure of DB and its rich negatively charged surface provided penetration anchor points for HBC molecular chains, which significantly enhanced the mechanical properties of H/D. The gelation temperature, gelation time and mechanical strength could be easily regulated by change of the ratio of DB in composite hydrogel. The maximum storage modulus of HBC hydrogel was increased to 17 times after adding DB. H/D exhibited favorable blood compatibility (hemolysis rate < 5 %) and no cytotoxicity to L929 cells which are promising for the applications as tissue regeneration materials and wound dressing in biomedical fields.
由羟丁基壳聚糖（HBC）和硅藻生物硅（DB）组成的温敏水凝胶（H/D）是通过将 HBC 溶液在低温下与 DB 物理混合制备的。流变学测量证实，将 DB 掺入羟丁基壳聚糖中可以显著提高水凝胶的机械强度，而不会破坏其可逆相转变行为。DB 的分级多孔结构及其丰富的带负电荷的表面为 HBC 分子链提供了渗透锚固点，从而显著提高了 H/D 的机械性能。通过改变复合水凝胶中 DB 的比例，可以轻松调节凝胶温度、凝胶时间和机械强度。添加 DB 后，HBC 水凝胶的最大储能模量增加到 17 倍。H/D 表现出良好的血液相容性（溶血率 < 5%）和对 L929 细胞无细胞毒性，有望作为组织再生材料和伤口敷料在生物医学领域得到应用。
