College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong Province, China.
Qingdao Municipal Hospital, Qingdao 266003, Shandong Province, China.
Carbohydr Polym. 2017 Aug 15;170:80-88. doi: 10.1016/j.carbpol.2017.04.038. Epub 2017 Apr 21.
Many cell delivery matrices have been developed due to the low transplantation efficiency of cell therapy. In the present study, thermosensitive hydroxybutyl chitosan (HBC) hydrogels were prepared with different formulations' solvent (Dulbecco's modified eagle's medium/phosphate buffered saline [DMEM/PBS], 0:100, 30:70, 50:50, 70:30, 100:0 [v/v]). Their gelation temperature was raised with DMEM ratio increase (from 9.5°C to 20.9°C). Pore sizes of HBC hydrogels treated with high ionic strength solutions became smaller. HUVECs cultured with HBC hydrogels exhibited proliferation in high DMEM ratio groups, and the optimal solvent formulation was DMEM/PBS 70:30 (v/v). Upon exposure to PBS, HUVECs encapsulated in HBC hydrogels could survive better than that when seeded on the surface of HBC hydrogels. These results demonstrated that HBC hydrogel could be a potential cell delivery matrix for cell therapy applications, especially when DMEM proportion in solvent formulations was higher than 50% (DMEM/PBS>50:50 [v/v]).
由于细胞治疗的移植效率低，已经开发出许多细胞输送基质。在本研究中，制备了不同配方溶剂（DMEM/磷酸盐缓冲盐水[DMEM/PBS]，0:100、30:70、50:50、70:30、100:0[v/v]）的温敏羟丁基壳聚糖（HBC）水凝胶。随着 DMEM 比例的增加，水凝胶的胶凝温度升高（从 9.5°C 升高到 20.9°C）。用高离子强度溶液处理的 HBC 水凝胶的孔径变小。在高 DMEM 比例组中培养的 HUVECs 增殖，最佳溶剂配方为 DMEM/PBS 70:30（v/v）。暴露于 PBS 时，包封在 HBC 水凝胶中的 HUVECs 的存活率高于接种在 HBC 水凝胶表面的 HUVECs。这些结果表明，HBC 水凝胶可能是细胞治疗应用的潜在细胞输送基质，特别是当溶剂配方中的 DMEM 比例高于 50%（DMEM/PBS>50:50[v/v]）时。
