College of Marine Life Science, Ocean University of China, Qingdao, 266003, PR China.
Department of Pathology, Qingdao Municipal Hospital, Qingdao, 266011, PR China.
Int J Biol Macromol. 2017 Dec;105(Pt 1):566-574. doi: 10.1016/j.ijbiomac.2017.07.082. Epub 2017 Jul 18.
This work targeted to investigate the potential of thermo-responsive hydroxybutyl chitosan (HBC) hydrogel using as an embolic material for occlusion of selective blood vessels. HBC hydrogel was prepared via an etherification reaction between chitosan (CS) and 1, 2-butene oxide. The hydroxybutyl groups were introduced into CS backbone, which endowed HBC hydrogel with properties of porous structure, favorable hydrophilia and rapid sol-gel interconvertibility. The gelation temperatures and gelation time respectively decreased from 30.7°C to 11.5°C and 79.60±3.19s to 7.70±1.42s at 37°C, with HBC solutions viscoelasticity increased from 3.0% to 7.0%. HBC hydrogel exhibited noncytotoxic to mouse embryo fibroblasts (MEFs) and excellent hemocompatibility with red blood cells (RBCs). After injection HBC solution into rat renal arteries, HBC solution transformed into hydrogel and attached onto blood vessel inner wall tightly, giving immediate blood vessels embolization. Meanwhile, RBCs could aggregate around HBC hydrogel to form moderate coagulation, which was beneficial to avoid hydrogel migration with blood flow. Above results suggested that HBC hydrogel could be applied as a promising embolic agent for hemorrage in the interventional vascular embolization field.
这项工作旨在研究温敏性羟丁基壳聚糖（HBC）水凝胶作为栓塞材料选择性闭塞血管的潜力。HBC 水凝胶是通过壳聚糖（CS）与 1,2-环氧丁烷的醚化反应制备的。羟丁基基团被引入 CS 主链中，使 HBC 水凝胶具有多孔结构、良好的亲水性和快速溶胶-凝胶相互转化的特性。在 37°C 时，凝胶温度从 30.7°C 降低至 11.5°C，凝胶时间从 79.60±3.19s 缩短至 7.70±1.42s，HBC 溶液的粘弹性从 3.0%增加至 7.0%。HBC 水凝胶对小鼠胚胎成纤维细胞（MEFs）无细胞毒性，对红细胞（RBC）具有优异的血液相容性。将 HBC 溶液注入大鼠肾动脉后，HBC 溶液转化为水凝胶并紧密附着在血管内壁上，立即实现血管栓塞。同时，RBC 可以聚集在 HBC 水凝胶周围形成适度的凝结，这有利于避免水凝胶随血流迁移。上述结果表明，HBC 水凝胶可用作介入血管栓塞领域出血的一种有前途的栓塞剂。
