Umeå Centre for Molecular Medicine, Umeå University, Johan Bures väg 12, 901 87, Umeå, Sweden.
Department of Medical Biosciences, Umeå University, Umeå, Sweden.
Sci Rep. 2020 Oct 26;10(1):18246. doi: 10.1038/s41598-020-74616-6.
The possibility to assess pancreatic anatomy with microscopic resolution in three dimensions (3D) would significantly add to pathological analyses of disease processes. Pancreatic ductal adenocarcinoma (PDAC) has a bleak prognosis with over 90% of the patients dying within 5 years after diagnosis. Cure can be achieved by surgical resection, but the efficiency remains drearily low. Here we demonstrate a method that without prior immunohistochemical labelling provides insight into the 3D microenvironment and spread of PDAC and premalignant cysts in intact surgical biopsies. The method is based solely on the autofluorescent properties of the investigated tissues using optical projection tomography and/or light-sheet fluorescence microscopy. It does not interfere with subsequent histopathological analysis and may facilitate identification of tumor-free resection margins within hours. We further demonstrate how the developed approach can be used to assess individual volumes and numbers of the islets of Langerhans in unprecedently large biopsies of human pancreatic tissue, thus providing a new means by which remaining islet mass may be assessed in settings of diabetes. Generally, the method may provide a fast approach to provide new anatomical insight into pancreatic pathophysiology.
以微观分辨率评估胰腺解剖结构的三维（3D）可能性将极大地增加对疾病过程的病理分析。胰腺导管腺癌（PDAC）预后不佳，超过 90%的患者在诊断后 5 年内死亡。通过手术切除可以治愈，但效率仍然很低。在这里，我们展示了一种无需预先进行免疫组织化学标记即可深入了解 PDAC 和癌前囊肿在完整手术活检中的 3D 微环境和扩散的方法。该方法仅基于使用光学投影断层扫描和/或光片荧光显微镜对研究组织的自发荧光特性。它不会干扰随后的组织病理学分析，并可能有助于在数小时内识别无肿瘤的切除边缘。我们进一步展示了如何使用所开发的方法来评估人类胰腺组织中前所未有的大活检中胰岛的个体体积和数量，从而为糖尿病环境中评估剩余胰岛质量提供了一种新的方法。总的来说，该方法可能为快速提供新的胰腺生理学解剖见解提供了一种方法。
