State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Biomater Adv. 2022 Oct;141:213105. doi: 10.1016/j.bioadv.2022.213105. Epub 2022 Sep 6.
Hydrogels composed of natural biopolymers are attractive for tissue regeneration applications owing to their advantages such as biocompatibility and ease of administration, etc.. Yet, the low oxygen level and the crosslinked network inside bulk hydrogels, as well as the hypoxic status in defect areas, hamper cell viability, function, and eventual tissue repair. Herein, based on Ca-crosslinked alginate hydrogel, oxygen-generating calcium peroxide (CaO) was introduced, which could provide a dynamic crosslinking alongside the CaO decomposition. Compared to the CaCl-crosslinked alginate hydrogel, bone marrow mesenchymal stromal cells cultured with CaO-contained system displayed remarkably improved biological behaviors. Furthermore, in vivo evaluations were carried out on a subcutaneous implantation in rats, and the results demonstrated the importance of the local oxygen availability in a series of crucial events for tissue regeneration, such as activating cell viability, migration, angiogenesis, and osteogenesis. In summary, the obtained Ca-crosslinked alginate hydrogel achieved a better microenvironment for cell ingrowth and potential tissue regeneration as the CaCl crosslinker being replaced by oxygen-generating CaO nanoparticles, due to its contribution in remedying the local hypoxic condition, promisingly, the release of Ca makes the hydrogel to be a possible candidate scaffold for bone tissue engineering.
由天然生物聚合物组成的水凝胶由于其生物相容性和易于给药等优点，在组织再生应用中很有吸引力。然而，水凝胶本体中的低氧水平和交联网络，以及缺陷区域的缺氧状态，会阻碍细胞的活力、功能和最终的组织修复。在此，基于钙交联海藻酸钠水凝胶，引入了产氧的过氧化钙（CaO），它可以在 CaO 分解的同时提供动态交联。与用 CaCl2交联的海藻酸钠水凝胶相比，在含有 CaO 的体系中培养的骨髓间充质基质细胞表现出显著改善的生物学行为。此外，在大鼠皮下植入模型中进行了体内评价，结果表明局部氧可用性在组织再生的一系列关键事件中非常重要，如激活细胞活力、迁移、血管生成和成骨。总之，由于用产氧 CaO 纳米粒子替代 CaCl2 交联剂，所获得的 Ca 交联海藻酸钠水凝胶为细胞侵入和潜在的组织再生提供了更好的微环境，有望释放 Ca 使水凝胶成为骨组织工程的潜在候选支架。
