The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, V6T 1Z3, Canada.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
J Dent. 2022 Nov;126:104285. doi: 10.1016/j.jdent.2022.104285. Epub 2022 Sep 9.
To investigate whether the adhesive-dentin interface stability of primary teeth would be enhanced by epigallocatechin-3-gallate (EGCG) with ethanol wet-bonding.
Non-caries primary molars were sliced to achieve a flat dentin surface and etched then randomly distributed into five groups in accordance with different treatments: group 1, no treatment; group 2, applying absolute ethanol wet-bonding for 60 s; groups 3-5, applying 0.1%, 0.5%, and 1% (w/v) EGCG-incorporating ethanol wet-bonding (0.1%, 0.5%, and 1% EGCG) for 60 s. Singlebond universal adhesive was then applied followed by resin composite construction. Microtensile bond strength, fracture mode, and nanoleakage at adhesive-dentin interface were evaluated after 24 h of water storage or 10,000 times of thermocycling. Zymography of hybrid layer, biofilm formation of Streptococcus mutans by CLSM, FESEM, and MTT test, and cytotoxicity by CCK-8 assay were respectively assessed.
Irrespective of thermocycling, the dentin bond strength was preserved with reduced nanoleakage in the 0.5% and 1% EGCG groups. Furthermore, the activity of endogenous proteases and the growth of Streptococcus mutans biofilm were inhibited after treatment with 0.5% and 1% EGCG/ethanol solutions (groups 4 and 5). CCK-8 results of the 0.1% and 0.5% EGCG groups showed acceptable biocompatibility.
Treatment by EGCG/ethanol solutions effectively enhanced the bond stability of primary teeth at the adhesive-dentin interface.
Synergistic application of EGCG and ethanol wet-bonding suggesting a promising strategy to improve dentin bonding durability with bacterial biofilm inhibition, thus increasing resin-based restorations' service life in primary dentition.
研究表没食子儿茶素没食子酸酯（EGCG）与乙醇湿粘接是否能增强乳牙的牙本质粘结界面稳定性。
将无龋的乳牙切片以获得平坦的牙本质表面，然后酸蚀并根据不同的处理随机分为五组：第 1 组，不处理；第 2 组，应用无水乙醇湿粘接 60 s；第 3-5 组，应用 0.1%、0.5%和 1%（w/v）含 EGCG 的乙醇湿粘接（0.1%、0.5%和 1%EGCG）60 s。然后应用单组分通用粘结剂，再进行树脂复合材料构建。水储存 24 h 或热循环 10,000 次后，评估微拉伸粘结强度、粘结剂-牙本质界面的断裂模式和纳米渗漏，以及通过 Zymography 检测混合层、CLSM、FESEM 和 MTT 试验检测变形链球菌生物膜形成、CCK-8 法检测细胞毒性。
无论热循环如何，0.5%和 1%EGCG 组的牙本质粘结强度保持不变，纳米渗漏减少。此外，用 0.5%和 1%EGCG/乙醇溶液处理后，内源性蛋白酶的活性和变形链球菌生物膜的生长受到抑制（第 4 组和第 5 组）。0.1%和 0.5%EGCG 组的 CCK-8 结果显示出良好的生物相容性。
EGCG/乙醇溶液处理可有效增强乳牙粘结剂-牙本质界面的粘结稳定性。
EGCG 与乙醇湿粘接的协同应用为抑制细菌生物膜、提高牙本质粘结耐久性提供了一种有前景的策略，从而延长乳牙树脂修复体的使用寿命。
