The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
CR&WISCO General Hospital, Wuhan, China.
J Dent. 2019 Nov;90:103207. doi: 10.1016/j.jdent.2019.103207. Epub 2019 Oct 3.
This study aimed to investigate the potential of baicalein combined with ethanol-wet bonding (EWB) in improving dentin bonding durability.
Sixty caries-free human third molars were randomly allocated into four groups and pretreated with solutions after sectioning and polishing. The pretreatments were prepared via dissolving baicalein in ethanol at concentrations of 0, 0.01%, 0.05% and 0.1% (w/v). Microtensile bond strength (MTBS) test, failure mode analysis and interfacial nanoleakage evaluation were conducted immediately or after thermocycling or 1 month of collagenase aging. In situ zymography, contact angle, antibacterial activity and bioactivity were comprehensively assessed.
Results demonstrated that the three experimental groups exhibited higher MTBS and lower nanoleakage expression regardless of aging. MMP activity within hybrid layer and Streptococcus. mutans biofilm formation were inhibited in the experimental groups in a dose-dependent manner. Baicalein also reduced reactive oxygen species (ROS) expression in human dental pulp cells and resisted adhesive-induced cytotoxicity. Baicalein exhibited remarkable capabilities at concentrations higher than 0.05% (w/v).
Baicalein is a prospective candidate as bioactive dentin bonding agent. Combined with EWB, baicalein may form a functional bonding interface, thereby enhancing dentin bond strength and durability.
Joint efforts by baicalein and EWB provides a novel therapeutic strategy for obtaining ideal adhesive-dentin interface and prolonging the longevity of restorations.
本研究旨在探讨黄芩素联合乙醇湿粘接（EWB）提高牙本质粘接耐久性的潜力。
将 60 颗无龋人第三磨牙随机分为 4 组，在切割和抛光后用溶液进行预处理。将黄芩素溶于乙醇中，浓度分别为 0、0.01%、0.05%和 0.1%（w/v），制备预处理液。立即或热循环或胶原蛋白酶老化 1 个月后进行微拉伸粘接强度（MTBS）测试、失效模式分析和界面纳米渗漏评估。综合评估原位酶谱、接触角、抗菌活性和生物活性。
结果表明，无论老化情况如何，三个实验组的 MTBS 均较高，纳米渗漏表达均较低。实验组中混合层内 MMP 活性和变形链球菌生物膜形成呈剂量依赖性抑制。黄芩素还降低了人牙髓细胞中活性氧（ROS）的表达，并抵抗了粘接力诱导的细胞毒性。黄芩素在浓度高于 0.05%（w/v）时表现出显著的性能。
黄芩素是一种有前途的生物活性牙本质粘接剂候选物。与 EWB 联合使用时，黄芩素可能会形成功能性粘接界面，从而提高牙本质的粘接强度和耐久性。
黄芩素和 EWB 的联合作用为获得理想的粘接-牙本质界面和延长修复体寿命提供了一种新的治疗策略。
