黄芩素与乙醇湿粘接联合提高牙本质粘接耐久性。

Combination of baicalein and ethanol-wet-bonding improves dentin bonding durability.

机构信息

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

CR&WISCO General Hospital, Wuhan, China.

出版信息

J Dent. 2019 Nov;90:103207. doi: 10.1016/j.jdent.2019.103207. Epub 2019 Oct 3.

DOI:10.1016/j.jdent.2019.103207

PMID:31586587

Abstract

OBJECTIVES

This study aimed to investigate the potential of baicalein combined with ethanol-wet bonding (EWB) in improving dentin bonding durability.

METHODS

Sixty caries-free human third molars were randomly allocated into four groups and pretreated with solutions after sectioning and polishing. The pretreatments were prepared via dissolving baicalein in ethanol at concentrations of 0, 0.01%, 0.05% and 0.1% (w/v). Microtensile bond strength (MTBS) test, failure mode analysis and interfacial nanoleakage evaluation were conducted immediately or after thermocycling or 1 month of collagenase aging. In situ zymography, contact angle, antibacterial activity and bioactivity were comprehensively assessed.

RESULTS

Results demonstrated that the three experimental groups exhibited higher MTBS and lower nanoleakage expression regardless of aging. MMP activity within hybrid layer and Streptococcus. mutans biofilm formation were inhibited in the experimental groups in a dose-dependent manner. Baicalein also reduced reactive oxygen species (ROS) expression in human dental pulp cells and resisted adhesive-induced cytotoxicity. Baicalein exhibited remarkable capabilities at concentrations higher than 0.05% (w/v).

CONCLUSION

Baicalein is a prospective candidate as bioactive dentin bonding agent. Combined with EWB, baicalein may form a functional bonding interface, thereby enhancing dentin bond strength and durability.

SIGNIFICANCE

Joint efforts by baicalein and EWB provides a novel therapeutic strategy for obtaining ideal adhesive-dentin interface and prolonging the longevity of restorations.

摘要

目的

本研究旨在探讨黄芩素联合乙醇湿粘接（EWB）提高牙本质粘接耐久性的潜力。

方法

将 60 颗无龋人第三磨牙随机分为 4 组，在切割和抛光后用溶液进行预处理。将黄芩素溶于乙醇中，浓度分别为 0、0.01%、0.05%和 0.1%（w/v），制备预处理液。立即或热循环或胶原蛋白酶老化 1 个月后进行微拉伸粘接强度（MTBS）测试、失效模式分析和界面纳米渗漏评估。综合评估原位酶谱、接触角、抗菌活性和生物活性。