Lu Xiaoyan, Zhang Kexin, Wang Tianli, Zhang Xueqing, Zhang Jianghui, Wei Hongyuan, Gao Pan, Wang Jun, Zhang Hongbo, Zhang Zhihua
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, China.
Qingwei Public Health Service Center of Luyang, Hefei 230000, China.
Curr HIV Res. 2022;20(5):407-418. doi: 10.2174/1570162X20666220908105918.
Studies have found that HIV is mainly transmitted through the mucosal surface, and the entrance of early progression of the disease is the rectal and colonic mucosa. So, this paper aimed to explore and analyze the structural differences of gut microbiome between men who have sex with men (MSM) and those who don't have sex with men (Non-MSM), expecting to find novel biological factors that potentially impact transmission and/or disease in MSM population.
We collected a total of 33 stool samples, 16 were MSM and 17 were Non-MSM. The 16S rRNA gene amplification sequencing was used to detect the alteration and structure of the gut microbiome community in two groups.
The difference in β diversity of gut microbiome of two groups of subjects was statistically significant (P<0.001), indicating that the difference in the structure of the gut microbiome of two groups was statistically significant. Compared with the phylum and genus level of Non-MSM group, the relative abundances of Actinobacteria, Proteobacteria, genera Collinsella, Prevotella, Bifidobacterium and Ralstonia in MSM group were higher (P<0.001, P<0.05, LDA score (log10)>2), and the relative abundance of Bacteroidetes, genera Erysipelotrichaceae incertae sedis, Bilophila, Holdemania, Clostridium XIVb and Bacteroidaceae in MSM group were lower (P<0.01, LDA score (log10)>2).
There are some differences in the structure of gut microbiome between MSM group and Non-MSM group. It indicates the differences in behavior and characteristics between MSM and Non-MSM populations may be related to the difference in the structure of gut microbiome.
研究发现，HIV主要通过黏膜表面传播，疾病早期进展的入口是直肠和结肠黏膜。因此，本文旨在探索和分析男男性行为者（MSM）与非男男性行为者（非MSM）肠道微生物群的结构差异，期望找到可能影响MSM人群传播和/或疾病的新生物因素。
我们共收集了33份粪便样本，其中16份来自MSM，17份来自非MSM。采用16S rRNA基因扩增测序检测两组肠道微生物群落的变化和结构。
两组受试者肠道微生物群的β多样性差异具有统计学意义（P<0.001），表明两组肠道微生物群结构差异具有统计学意义。与非MSM组的门和属水平相比，MSM组中放线菌门、变形菌门、柯林斯菌属、普雷沃菌属、双歧杆菌属和罗尔斯通氏菌属的相对丰度较高（P<0.001，P<0.05，线性判别分析得分（log10）>2），而MSM组中拟杆菌门、不确定的丹毒丝菌科属、嗜胆菌属、霍尔德曼氏菌属、梭菌属XIVb和拟杆菌科的相对丰度较低（P<0.01，线性判别分析得分（log10）>2）。
MSM组和非MSM组肠道微生物群结构存在一些差异。这表明MSM与非MSM人群在行为和特征上的差异可能与肠道微生物群结构的差异有关。
