Weith A, Winking H, Brackmann B, Boldyreff B, Traut W
EMBO J. 1987 May;6(5):1295-300. doi: 10.1002/j.1460-2075.1987.tb02367.x.
The DNA sequence organization of a homogeneously staining region (HSR) in the germ line of Mus musculus was studied with DNA clones generated by microdissection and microcloning. Six HSR-derived microclones were selected and characterized by Southern blot hybridizations. Four represented single-copy mouse DNA sequences. They were amplified in the HSR as fragments co-migrating with the respective normal mouse sequence and as additional fragments of different mobilities. The copy number of co-migrating fragments was approximately 16 for each of the four sequences but the number of rearranged fragments varied. Two microclones contained DNA sequences not detectable in normal mouse genomes but present, and one of them amplified, in the HSR. The observations suggest that the HSR developed from a part of the mouse genome by alternating replication and rearrangement events, with a specific integration of putative foreign DNA sequences.
利用显微切割和微克隆产生的DNA克隆，对小家鼠生殖系中均匀染色区（HSR）的DNA序列组织进行了研究。选择了6个源自HSR的微克隆，并通过Southern印迹杂交进行表征。其中4个代表单拷贝小鼠DNA序列。它们在HSR中作为与各自正常小鼠序列共迁移的片段以及不同迁移率的其他片段进行扩增。这4个序列中每个共迁移片段的拷贝数约为16，但重排片段的数量有所不同。另外两个微克隆包含在正常小鼠基因组中无法检测到但在HSR中存在且其中一个被扩增的DNA序列。这些观察结果表明，HSR是由小鼠基因组的一部分通过交替的复制和重排事件发展而来的，其中假定的外源DNA序列发生了特异性整合。
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