Holtrup Sven, Greger Maximilian, Mayer Benjamin, Specht Mara, Waidner Barbara
LOEWE Center for Synthetic Microbiology, Marburg, Germany.
Department of Biochemistry and Chemistry, Philipps University of Marburg, Marburg, Germany.
Front Microbiol. 2022 Aug 24;13:929194. doi: 10.3389/fmicb.2022.929194. eCollection 2022.
One important factor that promotes the colonization of the upper digestive system of the human pathogen is its helical cell shape. The bacteria cell shape is predominantly defined by its peptidoglycan cell wall. In rod-shaped species, PG synthesis is mediated by two dynamic molecular machines that facilitate growth along the perpendicular axis and the septum, called the elongasome and the divisome, respectively. Furthermore, many bacteria evolved additional mechanisms to locally change PG synthesis patterns to generate diverse cell shapes. Recent work characterizing cell shape mutants of revealed a novel mechanism for the generation of a twisted helix from a rod, including PG-modifying enzymes as well as additional proteins such as the bactofilin homolog CcmA or the membrane proteins Csd5 and Csd7. In this study, we investigate the localization and dynamics of CcmA and Csd7 using live-cell imaging. We also address the question of how these change in the presence or absence of the putative interaction partners.
促进人类病原体在上消化道定殖的一个重要因素是其螺旋状细胞形态。细菌的细胞形态主要由其肽聚糖细胞壁决定。在杆状菌中，肽聚糖（PG）合成由两种动态分子机器介导，这两种分子机器分别促进沿垂直轴和隔膜的生长，分别称为伸长体和分裂体。此外，许多细菌进化出了额外的机制来局部改变PG合成模式，以产生不同的细胞形态。最近对[具体细菌名称]细胞形态突变体的研究揭示了一种从杆状产生扭曲螺旋的新机制，包括PG修饰酶以及其他蛋白质，如细菌肌动蛋白同源物CcmA或膜蛋白Csd5和Csd7。在本研究中，我们使用活细胞成像技术研究了CcmA和Csd7的定位和动态变化。我们还探讨了在假定的相互作用伙伴存在或不存在的情况下，这些是如何变化的问题。
