da Silva Danilo Couto, Vaz Leonardo Gomes, Tavares Warley Luciano Fonseca, Vieira Leda Quercia, de Oliveira Ricardo Reis, Sobrinho Antônio Paulino Ribeiro
Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Restor Dent Endod. 2022 Jul 14;47(3):e31. doi: 10.5395/rde.2022.47.e31. eCollection 2022 Aug.
This study aimed to evaluate the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.
Cell viability assays, cell adherence, yeast phagocytosis of and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not.
After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS.
Our results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.
本研究旨在评估自粘树脂水门汀RelyX U200（3M ESPE）和seT PP（SDI Limited）对小鼠巨噬细胞的影响以及光活化的干扰作用。
在存在含有各自自粘水门汀的毛细管的情况下，进行细胞活力测定、细胞黏附、酵母吞噬和活性氧（ROS）生成实验，无论是否进行光活化。
长时间接触后，两种水门汀在未光活化时对巨噬细胞的细胞毒性更大。仅化学活化的seT PP水门汀似乎对酵母吞噬过程的负面影响更大。两种水门汀均会干扰细胞黏附过程，与光活化无关。所测试的任何一种水门汀类型均不能诱导ROS生成。
我们的结果凸显了牙科临床中自粘树脂水门汀光活化的重要性，因为光活化后的RelyX U200在所评估参数范围内表现出最佳结果。
