Merli M, Sacchini D, Iapichino S, Anzà M, Berto E, Masella R, Mantovani A, Riggio O, Cantafora A
Eur Surg Res. 1987;19(3):151-8. doi: 10.1159/000128694.
This study was designed to determine the individual fatty-acid pattern in plasma and in two adipose tissue sites of rats submitted to portacaval shunt (PCS) using the button technique. The animals were studied both in a fed state and after a 48-hour fast. Glucose, ketone bodies (KB), lactate and pyruvate levels were also measured. The results of the shunted animals were compared with pair-fed and freely fed control groups. In PCS rats, plasma free fatty-acid (FFA) levels were not significantly higher than in freely fed controls. beta-Hydroxybutyrate was significantly lower in PCS rats compared with both control groups. A 48-hour fast caused an elevation of plasma FFA and KB in all groups, but in PCS rats this increase was less pronounced. Lactate levels did not decrease during fasting in PCS rats, as they did in the control groups. PCS did not modify plasma FFA or the adipose tissue composition, neither in fed nor in fasted rats. We conclude that the PCS rats operated upon by using the button technique show mild modifications in lipid metabolism, affecting the fatty-acid composition neither in plasma FFA nor in adipose tissue.
本研究旨在采用纽扣技术确定接受门腔分流术（PCS）的大鼠血浆及两个脂肪组织部位的个体脂肪酸模式。对动物在进食状态及禁食48小时后均进行了研究。还测定了葡萄糖、酮体（KB）、乳酸和丙酮酸水平。将分流动物的结果与配对喂养和自由喂养的对照组进行比较。在PCS大鼠中，血浆游离脂肪酸（FFA）水平并不显著高于自由喂养的对照组。与两个对照组相比，PCS大鼠的β-羟基丁酸水平显著降低。禁食48小时导致所有组的血浆FFA和KB升高，但在PCS大鼠中这种升高不太明显。在禁食期间，PCS大鼠的乳酸水平不像对照组那样下降。无论是在进食的大鼠还是禁食的大鼠中，PCS均未改变血浆FFA或脂肪组织组成。我们得出结论，采用纽扣技术进行手术的PCS大鼠在脂质代谢方面表现出轻微改变，既未影响血浆FFA中的脂肪酸组成，也未影响脂肪组织中的脂肪酸组成。
