Lynch J W, Miles J M, Bailey J W
Department of Nutrition, University of Tennessee, Knoxville 37996.
JPEN J Parenter Enteral Nutr. 1994 May-Jun;18(3):208-13. doi: 10.1177/0148607194018003208.
Diets containing either triacetin (the water-soluble triglyceride of acetate) or long-chain triglycerides (LCTs) were fed to rats to determine the effects on intestinal mucosa cells and plasma substrates. Male Sprague-Dawley rats were fed one of three diets, a control diet containing 5% of energy as LCTs or one of two experimental diets that contained 30% of energy as lipid. The lipid component of the two experimental diets was either 100% LCTs or 95% triacetin/5% LCTs. Plasma lactate, glucose, and total ketone body concentrations were not significantly different among dietary treatment groups. Compared with animals fed LCTs and control diet, plasma pyruvate and free fatty acid concentrations were decreased in animals fed triacetin. In contrast, plasma triglyceride concentrations were elevated in animals fed triacetin compared with other groups. Intestinal biochemical measures included total DNA, RNA, protein, and the protein:DNA ratio. Histologic indices measured were villus height in the jejunum and crypt depth in the colon. No significant difference in mucosal protein concentration was observed in the jejunum and colon. Jejunal RNA was significantly decreased in animals fed triacetin compared with other diets. Triacetin feeding significantly increased the DNA content in the jejunum and colon (thereby lowering the protein:DNA ratio), indicating smaller, more numerous cells. Jejunal villus height and colonic crypt depth were not significantly different among dietary treatment groups. Provision of a balanced diet containing 28.5% of the total calories as triacetin had no adverse effects on metabolic substrates and resulted in smaller and more numerous mucosal cells in the jejunum and colon.(ABSTRACT TRUNCATED AT 250 WORDS)
给大鼠喂食含有三醋精（醋酸的水溶性甘油三酯）或长链甘油三酯（LCT）的饮食，以确定其对肠黏膜细胞和血浆底物的影响。将雄性斯普拉格-道利大鼠分为三组喂食，一组为对照饮食，含5%能量的LCT，另外两组为实验饮食，含30%能量的脂质。两种实验饮食的脂质成分分别为100%LCT或95%三醋精/5%LCT。各饮食处理组间血浆乳酸、葡萄糖和总酮体浓度无显著差异。与喂食LCT和对照饮食的动物相比，喂食三醋精的动物血浆丙酮酸和游离脂肪酸浓度降低。相反，与其他组相比，喂食三醋精的动物血浆甘油三酯浓度升高。肠道生化指标包括总DNA、RNA、蛋白质以及蛋白质与DNA的比率。所测量的组织学指标为空肠绒毛高度和结肠隐窝深度。空肠和结肠黏膜蛋白浓度未观察到显著差异。与其他饮食相比，喂食三醋精的动物空肠RNA显著降低。喂食三醋精显著增加了空肠和结肠中的DNA含量（从而降低了蛋白质与DNA的比率），表明细胞更小、数量更多。各饮食处理组间空肠绒毛高度和结肠隐窝深度无显著差异。提供一种含28.5%总热量为三醋精的均衡饮食对代谢底物没有不良影响，并导致空肠和结肠中黏膜细胞更小、数量更多。（摘要截短至250字）
