Prasad Priya
Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai, India.
Present Address: Department of Biotechnology, National Institute of Technology, Warangal, Telangana India.
3 Biotech. 2022 Oct;12(10):263. doi: 10.1007/s13205-022-03341-w. Epub 2022 Sep 6.
RSC (Remodel the Structure of Chromatin) chromatin remodeler in has multiple subunits, some of them are essential whereas the others are non-essential for cell viability. Not all the subunits are involved in all the functions of RSC complex. With several lines of evidences showing somewhat similar role of RSC complex [through Sth1 (Snf Two Homolog 1)] in different aspects of cell cycle (chromosome segregation and kinetochore function, etc.), DNA damage repair and stress response in and , we hypothesize the organization of RSC complex in could follow the same pattern as observed in . We hypothesize that the subset of the subunits could form different subcomplexes to do different biological functions where several subunits are common among the subcomplexes. We wished to address if the accessory component Rsc9 protein of RSC complex has any non-overlapping function with respect to the ATPase component Sth1 of the RSC complex in . Here, we have shown that the mutant exhibit abnormal nuclear segregation, though budding profile is comparable to the wild type. Rsc9 does not affect yeast to hyphae transition. Though both Sth1 and Rsc9 belong to the same RSC complex, individual protein is functionally distinct, and the respective mutant shows different phenotype and different consequence in cellular process. This suggests that though RSC works as a single complex having global remodeling activity, different subunit can form distinct modules which show functional disparity, as the components show some similar and some distinct function.
The online version contains supplementary material available at 10.1007/s13205-022-03341-w.
酿酒酵母中的RSC（重塑染色质结构）染色质重塑因子有多个亚基，其中一些对细胞活力至关重要，而其他亚基则非必需。并非所有亚基都参与RSC复合物的所有功能。有几条证据表明，RSC复合物[通过Sth1（Snf2同源物1）]在酿酒酵母细胞周期的不同方面（染色体分离和动粒功能等）、DNA损伤修复和应激反应中发挥着 somewhat 相似的作用，我们推测酿酒酵母中RSC复合物的组织方式可能与裂殖酵母中观察到的模式相同。我们假设亚基的子集可以形成不同的亚复合物来执行不同的生物学功能，其中几个亚基在亚复合物之间是共同的。我们希望探讨RSC复合物的辅助成分Rsc9蛋白相对于裂殖酵母中RSC复合物的ATP酶成分Sth1是否具有任何非重叠功能。在这里，我们已经表明，该突变体表现出异常的核分离，尽管出芽情况与野生型相当。Rsc9不影响酵母向菌丝的转变。虽然Sth1和Rsc9都属于同一个RSC复合物，但单个蛋白质在功能上是不同的，各自的突变体在细胞过程中表现出不同的表型和不同的结果。这表明，尽管RSC作为一个具有全局重塑活性的单一复合物起作用，但不同的亚基可以形成不同的模块，这些模块表现出功能差异，因为这些成分表现出一些相似和一些不同的功能。
在线版本包含可在10.1007/s13205-022-03341-w获取的补充材料。
