Khan Hanif, Krishnappa Gopalareddy, Kumar Satish, Mishra Chandra Nath, Krishna Hari, Devate Narayana Bhat, Rathan Nagenahalli Dharmegowda, Parkash Om, Yadav Sonu Singh, Srivastava Puja, Biradar Suma, Kumar Monu, Singh Gyanendra Pratap
ICAR-Indian Institute of Wheat and Barley Research, Karnal, India.
ICAR-Sugarcane Breeding Institute, Coimbatore, India.
Front Genet. 2022 Aug 26;13:982589. doi: 10.3389/fgene.2022.982589. eCollection 2022.
Genomic regions governing days to heading (DH), grain filling duration (GFD), grain number per spike (GNPS), grain weight per spike (GWPS), plant height (PH), and grain yield (GY) were investigated in a set of 280 diverse bread wheat genotypes. The genome-wide association studies (GWAS) panel was genotyped using a 35K Axiom Array and phenotyped in five environments. The GWAS analysis showed a total of 27 Bonferroni-corrected marker-trait associations (MTAs) on 15 chromosomes representing all three wheat subgenomes. The GFD showed the highest MTAs (8), followed by GWPS (7), GY (4), GNPS (3), PH (3), and DH (2). Furthermore, 20 MTAs were identified with more than 10% phenotypic variation. A total of five stable MTAs (, and ) were identified in more than one environment and associated with the expression of DH, GFD, GNPS, and GY. Similarly, two novel pleiotropic genomic regions with associated MTAs i.e. (4D) and (6A) harboring co-localized QTLs governing two or more traits were also identified. analysis revealed that the SNPs were located on important putative candidate genes such as , , , , involved in the regulation of grain size, grain number, growth and development, grain filling duration, and abiotic stress tolerance. The identified novel MTAs will be validated to estimate their effects in different genetic backgrounds for subsequent use in marker-assisted selection (MAS).
在一组280个不同的面包小麦基因型中，研究了控制抽穗天数（DH）、灌浆持续时间（GFD）、每穗粒数（GNPS）、每穗粒重（GWPS）、株高（PH）和籽粒产量（GY）的基因组区域。使用35K Axiom芯片对全基因组关联研究（GWAS）群体进行基因分型，并在五个环境中进行表型分析。GWAS分析显示，在代表所有三个小麦亚基因组的15条染色体上共有27个经Bonferroni校正的标记-性状关联（MTA）。GFD的MTA数量最多（8个），其次是GWPS（7个）、GY（4个）、GNPS（3个）、PH（3个）和DH（2个）。此外，还鉴定出20个表型变异超过10%的MTA。总共在多个环境中鉴定出5个稳定的MTA（……），它们与DH、GFD、GNPS和GY的表达相关。同样，还鉴定出两个具有相关MTA的新的多效性基因组区域，即位于4D染色体上的……和位于6A染色体上的……，它们含有共同定位的控制两个或更多性状的QTL。……分析表明，这些单核苷酸多态性（SNP）位于重要的假定候选基因上，如……，这些基因参与调控籽粒大小、粒数、生长发育、灌浆持续时间和非生物胁迫耐受性。所鉴定的新MTA将进行验证，以评估它们在不同遗传背景下的效应，供后续用于标记辅助选择（MAS）。
