Wang Yu, Mei Li, Zhao Shuya, Xing Xianghui, Wu Guofeng
Department of Pediatric Dentistry, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
Discipline of Orthodontics, Department of Oral Science, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
Technol Health Care. 2023;31(2):647-659. doi: 10.3233/THC-220195.
The integrity and stability of collagen are crucial for the dentin structure and bonding strength at dentin-resin interface. Natural plant-derived polypehenols have been used as collagen crosslinkers.
The aims of the study were to develop novel chitosan oleuropein nanoparticles (CS-OL-NPs), and to investigate the CS-OL-NPs treated dentin's the resistance to enzymatic degradation and mechanic property.
CS-OL-NPs were developed using the ionotropic gelation method. Release and biocompatibility of the CS-OL-NPs were tested. Twenty demineralized dentin collage specimens were randomized into four interventions groups: A, Deionized Water (DW); B, 5% glutaraldehyde solution (GA); C, 1 mg/ml chitosan (CS); and D, 100 mg/L CS-OL-NPs. After 1-min interventions, dentin matrix were evaluated by the micro-Raman spectroscopy for the modulus of elasticity test. Collagen degradation was assessed using hydroxyproline (HYP) assay.
CS-OL-NPs were spherical core-shape with a size of 161.29 ± 8.19 nm and Zeta potential of 19.53 ± 0.26 mV. After a burst release of oleuropein in the initial 6 h, there was a long-lasting steady slow release. CS-OL-NPs showed a good biocompatibility for the hPDLSCs. The modulus of elasticity in the crosslinked groups were significantly higher than that in the control group (P< 0.05 for all). The specimens treated with CS-OL-NP showed a greater modulus of elasticity than those treated with GA and CS (P< 0.05 for both). The release of HYP in the crosslinked group was significantly lower than that in the non-crosslinked groups (P< 0.05 for all).
CS-OL-NPs enhanced the dentin mechanical property and resistance to biodegradation, with biocompatibility and potential for clinical application.
胶原蛋白的完整性和稳定性对于牙本质结构以及牙本质-树脂界面的粘结强度至关重要。天然植物来源的多酚类物质已被用作胶原蛋白交联剂。
本研究旨在制备新型壳聚糖橄榄苦苷纳米粒（CS-OL-NPs），并研究经CS-OL-NPs处理的牙本质的抗酶降解能力和力学性能。
采用离子凝胶法制备CS-OL-NPs。测试CS-OL-NPs的释放情况和生物相容性。将20个脱矿牙本质胶原标本随机分为4个干预组：A组，去离子水（DW）；B组，5%戊二醛溶液（GA）；C组，1mg/ml壳聚糖（CS）；D组，100mg/L CS-OL-NPs。干预1分钟后，通过显微拉曼光谱评估牙本质基质的弹性模量进行测试。使用羟脯氨酸（HYP）测定法评估胶原蛋白降解情况。
CS-OL-NPs呈球形核状，尺寸为161.29±8.19nm，Zeta电位为19.53±0.26mV。在最初6小时橄榄苦苷突发释放后，有持久稳定的缓慢释放。CS-OL-NPs对人牙周膜干细胞显示出良好的生物相容性。交联组的弹性模量显著高于对照组（所有P<0.05）。经CS-OL-NP处理的标本显示出比经GA和CS处理的标本更大的弹性模量（两者P<0.05）。交联组中HYP的释放显著低于非交联组（所有P<0.05）。
CS-OL-NPs增强了牙本质的力学性能和抗生物降解能力，具有生物相容性和临床应用潜力。
