Hu Die, Tian Tian, Ren Qian, Han Sili, Li Zhongcheng, Deng Yudi, Lu Ziqian, Zhang Linglin
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China; Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China.
Dent Mater. 2024 Feb;40(2):160-172. doi: 10.1016/j.dental.2023.11.003. Epub 2023 Nov 10.
This study aims to synthesize novel chitosan nanoparticles loaded with an amelogenin-derived peptide QP5 (TMC-QP5/NPs), investigate their remineralization capability and inhibitory effects on endogenous matrix metalloproteinases (MMPs), and evaluate the dentin bonding properties of remineralized dentin regulated by TMC-QP5/NPs.
TMC-QP5/NPs were prepared by ionic crosslinking method and characterized by dynamic light scattering method, scanning electron microscopy, transmission electron microscope, atomic force microscope, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The encapsulation and loading efficiency of TMC-QP5/NPs and the release of QP5 were examined. To evaluate the remineralization capability of TMC-QP5/NPs, the mechanical properties, and the changes in structure and composition of differently conditioned dentin were characterized. The MMPs inhibitory effects of TMC-QP5/NPs were explored by MMP Activity Assay and in-situ zymography. The dentin bonding performance was detected by interfacial microleakage and microshear bond strength (μSBS).
TMC-QP5/NPs were successfully synthesized, with uniform size, good stability and biosafety. The encapsulation and loading efficiency of TMC-QP5/NPs was respectively 69.63 ± 2.22% and 13.21 ± 0.73%, with a sustained release of QP5. TMC-QP5/NPs could induce mineral deposits on demineralized collagen fibers and partial occlusion of dentin tubules, and recover the surface microhardness of dentin, showing better remineralization effects than QP5. Besides, TMC-QP5/NPs significantly inhibited the endogenous MMPs activity. The remineralized dentin induced by TMC-QP5/NPs exhibited less interfacial microleakage and higher μSBS, greatly improved dentin bonding.
This novel peptide-loaded chitosan nanoparticles improved resin-dentin bonding by promoting dentin remineralization and inactivating MMPs, suggesting a promising strategy for optimizing dentin adhesive restorations.
本研究旨在合成负载牙釉蛋白衍生肽QP5的新型壳聚糖纳米粒（TMC-QP5/NPs），研究其再矿化能力和对内源性基质金属蛋白酶（MMPs）的抑制作用，并评估TMC-QP5/NPs调控的再矿化牙本质的牙本质粘结性能。
采用离子交联法制备TMC-QP5/NPs，并通过动态光散射法、扫描电子显微镜、透射电子显微镜、原子力显微镜、傅里叶变换红外光谱和差示扫描量热法对其进行表征。检测TMC-QP5/NPs的包封率、载药率及QP5的释放情况。为评估TMC-QP5/NPs的再矿化能力，对不同处理的牙本质的力学性能、结构和组成变化进行表征。通过MMP活性测定和原位酶谱法探究TMC-QP5/NPs对MMPs的抑制作用。通过界面微渗漏和微拉伸粘结强度（μSBS）检测牙本质粘结性能。
成功合成了TMC-QP5/NPs，其尺寸均匀、稳定性好且具有生物安全性。TMC-QP5/NPs的包封率和载药率分别为69.63±2.22%和13.21±0.73%，QP5可缓释。TMC-QP5/NPs可在脱矿的胶原纤维上诱导矿物质沉积并部分封闭牙本质小管，恢复牙本质的表面显微硬度，显示出比QP5更好的再矿化效果。此外，TMC-QP5/NPs显著抑制内源性MMPs活性。TMC-QP5/NPs诱导的再矿化牙本质表现出更少的界面微渗漏和更高的μSBS，大大改善了牙本质粘结。
这种新型载肽壳聚糖纳米粒通过促进牙本质再矿化和使MMPs失活改善了树脂-牙本质粘结，为优化牙本质粘结修复提供了一种有前景的策略。
