Parinaud J, Monrozies X, Reme J M, Sarramon M F, Grandjean H, Pontonnier G
Eur J Obstet Gynecol Reprod Biol. 1987 Jul;25(3):203-8. doi: 10.1016/0028-2243(87)90100-6.
Culture media are important components of IVF. Their selection must meet the need for efficiency, and also economic and practical requirements. From these standpoints, we compared two widely used media: Ham's F10 supplemented with cord serum and Menezo B2 used without serum. The test followed a randomized protocol using two series of 159 and 162 oocytes. Since no difference was seen in efficiency assessed from cleavage rate, the discussion focuses on cost and ease of use.
培养基是体外受精的重要组成部分。其选择必须满足效率需求,同时还要符合经济和实用要求。从这些角度出发,我们比较了两种广泛使用的培养基:添加脐带血清的哈姆氏F10培养基和不添加血清使用的梅内佐B2培养基。试验遵循随机方案,使用了两组分别为159个和162个卵母细胞。由于从卵裂率评估的效率上未发现差异,因此讨论集中在成本和易用性上。