Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad #3000, Colonia, C.U., Coyoacán, 04510, Ciudad de Mexico, Mexico.
Departamento de Medicina Cirugía y Zootecnia Para Pequeñas Especies, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad #3000, Colonia, C.U., Coyoacán, 04510, Ciudad de Mexico, Mexico.
Arch Microbiol. 2022 Sep 12;204(10):616. doi: 10.1007/s00203-022-03227-8.
Ehrlichia canis has gained importance over the years as a zoonotic bacterium, nevertheless in Mexico is unknown the extent of the problem in animals and public health. The country had a few studies carried out locally using serology and molecular tests as diagnostic methods. Ehrlichiosis is not considered endemic in the central valley of Mexico, because the climatic conditions in the region have not allowed the vector (Rhipicephalus sanguineus) to establish itself adequately, therefore, diagnosis is not used in clinical practice in this area. A nested PCR (nPCR) offers rapid results with high sensitivity and specificity regardless of cost. The use of a recombinant positive control provides the advantage of timely diagnosis, follow-up treatment and allows the clinician to decide. In this work, the nPCR reported by Wen et al. (J Clin Microbiol 35(7):1852-2185, 1997) was used for the diagnosis of E. canis by modifying the reaction conditions to improve the detection of the test. We constructed a recombinant positive control to nPCR as diagnostic technique for E. canis, also we modified the reaction conditions to improve detection of the test which allowed the diagnosis of E. canis in dogs in the Mexican Republic using 53 samples from dogs with positive serological diagnosis of Ehrlichiosis, some of them from the valley of Mexico. Currently, this nPCR is offered to public at the Faculty of Veterinary Medicine and Zootechnics of the National Autonomous University of Mexico at an accessible cost and allows to begin to generate epidemiological information to know distribution of the bacterium.
犬埃立克体已成为一种重要的人畜共患病细菌，然而在墨西哥，动物和公共卫生方面的问题的严重程度尚不清楚。该国曾有一些使用血清学和分子检测作为诊断方法的局部研究。埃立克体病在墨西哥中央山谷并不被认为是地方性疾病，因为该地区的气候条件不允许媒介（血红扇头蜱）充分建立，因此，在该地区的临床实践中不使用诊断。巢式 PCR（nPCR）具有快速、高灵敏度和特异性的特点，无论成本如何。使用重组阳性对照具有及时诊断、后续治疗的优势，并允许临床医生做出决定。在这项工作中，使用了 Wen 等人报道的 nPCR（J Clin Microbiol 35(7):1852-2185, 1997），通过修改反应条件来提高测试的检测，用于诊断犬埃立克体。我们构建了一个重组阳性对照 nPCR 作为犬埃立克体的诊断技术，还修改了反应条件以提高测试的检测，这使得我们能够使用来自血清学诊断为埃立希体病的狗的 53 个样本，其中一些来自墨西哥山谷，在墨西哥共和国诊断犬埃立克体。目前，这种 nPCR 在墨西哥国立自治大学兽医学院和动物科学系以可负担的价格提供给公众，并允许开始生成流行病学信息以了解细菌的分布。
