Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):553-559. doi: 10.1007/s10517-022-05588-y. Epub 2022 Sep 12.
In squamous cell carcinoma of the larynx, the population of epithelial cells in the tumor tissue is initially heterogeneous and, in addition to tumor cells invading the organ mucosa, includes normal epithelial cells of protein-mucous glands and cells of the stratified epithelium covering the mucous membrane. A search for differential markers to separate these subpopulations was carried out. The surface marker CD44 and cytokeratins 5 and 17 that are often used to verify carcinoma cells, are common markers for all epithelial cells of the larynx. In highly differentiated carcinoma, subpopulations of normal and tumor epithelial cells can be separated by the level of expression of cytokeratins 10 and 18 and nuclear markers Ki-67 and p63. However, in moderately differentiated carcinoma, tumor cells and normal cells of the basal layer of the stratified epithelium covering the mucous membrane of the larynx have similar phenotypes, which should be taken into account when conducting experimental studies.
在喉鳞状细胞癌中，肿瘤组织中的上皮细胞群体最初是异质的，除了浸润器官黏膜的肿瘤细胞外，还包括蛋白黏液腺的正常上皮细胞和覆盖黏膜的复层上皮细胞。为了分离这些亚群，进行了寻找差异标志物的研究。表面标志物 CD44 和细胞角蛋白 5 和 17 常用于验证癌细胞，是喉所有上皮细胞的常见标志物。在高度分化的癌中，可以通过细胞角蛋白 10 和 18 的表达水平以及核标志物 Ki-67 和 p63 来分离正常和肿瘤上皮细胞的亚群。然而，在中度分化的癌中，肿瘤细胞和覆盖喉黏膜的复层上皮细胞的基底层的正常细胞具有相似的表型，在进行实验研究时应考虑到这一点。
