癌症患者连续射精用于精子冷冻保存时的精子DNA碎片情况。
Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation.
作者信息
Kim Seul Ki, Paik Haerin, Lee Jung Ryeol, Jee Byung Chul
机构信息
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea.
出版信息
Clin Exp Reprod Med. 2022 Sep;49(3):196-201. doi: 10.5653/cerm.2022.05323. Epub 2022 Aug 31.
OBJECTIVE
This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer.
METHODS
Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within- and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance.
RESULTS
The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45-0.84) and semen volume (ICC, 0.67; 95% CI, 0.45-0.84).
CONCLUSION
Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.
目的
本前瞻性连续研究调查了癌症患者多次精液样本中精子DNA碎片化（SDF）的变化情况。
方法
81例患有各种癌症的患者在癌症治疗前连续3天进行多次精液采集以用于精子冷冻保存。使用商用Halosperm试剂盒测量SDF。通过方差的随机效应分析估计受试者内和受试者间的变异系数，以评估精液参数和SDF的一致性。计算组内相关系数（ICC）以评估受试者间方差成分相对于总方差的大小。
结果
第2天和第3天样本的精液量与第1天样本相比显著降低。大多数参数显示出较高的ICC值，表明受试者内波动相对于受试者间变异性较小。SDF（ICC，0.68；95%置信区间[CI]，0.45 - 0.84）和精液量（ICC，0.67；95%CI，0.45 - 0.84）的ICC值最高。
结论
我们的研究结果表明癌症患者的多次射精具有稳定的SDF水平。
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