Meyer H, Ropp S L, Esposito J J
Institute of Microbiology, Federal Armed Forces Medical Academy, Munich, Germany.
J Virol Methods. 1997 Mar;64(2):217-21. doi: 10.1016/s0166-0934(96)02155-6.
Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox, variola, monkeypox, camelpox, ectromelia and vaccinia viruses) and 3 New World (skunkpox, volepox, and raccoonpox) resulted in amplicons that ranged in size from 510 to 1673 base pairs depending on the species, except for raccoonpox virus DNA which did not amplify. XbaI digest gel electrophoresis profiles of the amplicons improved resolution of the differences.
基于编码牛痘病毒嗜酸性包涵体（ATI）主要蛋白质成分的序列，使用单一引物对通过聚合酶链反应扩增基因组DNA来鉴定和区分正痘病毒种类。来自8种旧大陆病毒（牛痘、天花、猴痘、骆驼痘、鼠痘和痘苗病毒）中的6种以及3种新大陆病毒（臭鼬痘病毒、田鼠痘病毒和浣熊痘病毒）的可用DNA产生了大小在510至1673个碱基对之间的扩增子，具体大小取决于病毒种类，但浣熊痘病毒DNA未扩增。扩增子的XbaI酶切凝胶电泳图谱提高了差异的分辨率。
