兔模型中压缩机械应力下髁突吸收的研究-基质金属蛋白酶的相关性。
Condylar Resorption Following Compressive Mechanical Stress in Rabbit Model - Association of Matrix Metalloproteinases.
机构信息
Division of Oral and Maxillofacial Surgery, Department of Disease Management Dentistry, Tohoku University Graduate School of Dentistry, Miyagi, Japan;
Division of Oral and Maxillofacial Surgery, Department of Disease Management Dentistry, Tohoku University Graduate School of Dentistry, Miyagi, Japan.
出版信息
In Vivo. 2022 Sep-Oct;36(5):2126-2133. doi: 10.21873/invivo.12938.
BACKGROUND/AIM: Idiopathic condylar resorption (ICR) is a morphological change of the condylar head that occurs following orthodontic treatment or orthognathic surgery. This complication is serious, as it can cause relapse after mandible treatment. The aim of this experimental study was to evaluate the mechanism of influence of condylar resorption on compressive mechanical stress in temporomandibular joint following a change in occlusal position by mandible advancement.
MATERIALS AND METHODS
An osteotomy procedure at the midline of mandible was performed in 15 rabbits, with the left side moved forward by 3.5 mm. Advancement of the left side of the mandible resulted in compressive mechanical stress on condylar head on the left side. Samples were subjected to micro-computed tomography, histological staining and immunohistochemistry.
RESULTS
The area and depth of anterior condylar resorption at two weeks were significantly different as compared to those at one week (p<0.05). TRAP staining confirmed the significantly largest number of TRAP-positive cells after two weeks (p=0.02), compared to one week. MMP-3 and MMP-13 immunostaining of the anterior condylar head at two weeks revealed high levels of both proteins from the surface to the deep layer of cartilage.
CONCLUSION
Compressive mechanical stress following mandible advancement results in load on the anterior surface of the condylar head, which leads to bone resorption there, and induces MMP-3 and MMP-13 related to degradation of condylar head cartilage.
背景/目的：特发性髁突吸收（ICR）是一种在正畸治疗或正颌手术后发生的髁突头的形态变化。这种并发症很严重，因为它会导致下颌骨治疗后复发。本实验研究的目的是通过下颌骨前伸来评估咬合位置改变后髁突吸收对颞下颌关节压缩机械应力的影响机制。
材料和方法
在 15 只兔子的下颌骨中线进行了截骨术，将左侧向前移动 3.5 毫米。左侧下颌骨的前进导致左侧髁突头承受压缩机械应力。对样本进行了微计算机断层扫描、组织学染色和免疫组织化学分析。
结果
与一周相比，两周时前髁突吸收的面积和深度有显著差异（p<0.05）。TRAP 染色证实，与一周相比，两周后 TRAP 阳性细胞的数量明显最多（p=0.02）。两周时前髁突头的 MMP-3 和 MMP-13 免疫染色显示，两种蛋白从表面到软骨深层的水平均较高。
结论
下颌骨前伸后产生的压缩机械应力导致髁突头前表面承受负荷，导致该处骨吸收，并诱导与髁突头软骨降解相关的 MMP-3 和 MMP-13。
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