阿米替林对大鼠口腔微生物的全身给药作用。
Effect of Systemic Administration of Amitriptyline on Oral Microbes in Rats.
机构信息
Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan.
Advanced Research Promotion Center, Health Sciences University of Hokkaido, Tōbetsu, Japan.
出版信息
In Vivo. 2022 Sep-Oct;36(5):2134-2142. doi: 10.21873/invivo.12939.
BACKGROUND/AIM: Amitriptyline is a major tricyclic antidepressant that is also used to relieve chronic orofacial pain. Recently, alterations in gut flora due to various antidepressants have been demonstrated. However, it remains unknown how antidepressants affect the oral environment, including microbiota and innate immunity. The aim of this study was to investigate the effects of amitriptyline on oral microflora and antimicrobial peptides.
MATERIALS AND METHODS
Sprague-Dawley rats were intraperitoneally injected with amitriptyline for 2 weeks. The DNA extracted from the oral swabs were used to perform 16SrRNA sequencing to evaluate the oral microbiome. Quantitative RT-PCR was performed to evaluate the mRNA levels of antimicrobial peptides in the buccal tissues.
RESULTS
No significant differences in salivary flow rates were observed between the amitriptyline and control groups. Taxonomic analysis showed significant alterations in bacteria such as Corynebacterium, Rothia, and Porphyromonas due to amitriptyline administration. The beta diversity showed significant differences between the amitriptyline and control groups. Additionally, the predicted metagenome functions were significantly different between the two groups. The mRNA expression levels of antimicrobial peptides in the amitriptyline group were significantly higher as compared to controls.
CONCLUSION
Systemic administration of amitriptyline may affect the oral environment, including oral microbes and innate immunity in the oral mucosa.
背景/目的：阿米替林是一种主要的三环类抗抑郁药，也用于缓解慢性口面疼痛。最近，由于各种抗抑郁药的作用，肠道菌群发生了改变。然而，抗抑郁药如何影响口腔环境，包括微生物群和先天免疫，目前还不清楚。本研究旨在探讨阿米替林对口腔微生物群和抗菌肽的影响。
材料和方法
Sprague-Dawley 大鼠腹腔内注射阿米替林 2 周。从口腔拭子中提取的 DNA 用于进行 16SrRNA 测序，以评估口腔微生物组。采用定量 RT-PCR 法检测颊组织中抗菌肽的 mRNA 水平。
结果
阿米替林组和对照组的唾液流速无显著差异。分类分析显示，由于阿米替林的给药，棒状杆菌、罗氏菌和卟啉单胞菌等细菌发生了显著改变。β多样性显示阿米替林组和对照组之间存在显著差异。此外，两组之间预测的宏基因组功能也有显著差异。与对照组相比，阿米替林组抗菌肽的 mRNA 表达水平显著升高。
结论
阿米替林的全身给药可能会影响口腔环境，包括口腔微生物和口腔黏膜的先天免疫。
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