小鼠模型中的口腔微生物组特征分析
Oral Microbiome Characterization in Murine Models.
作者信息
Abusleme Loreto, Hong Bo-Young, Hoare Anilei, Konkel Joanne E, Diaz Patricia I, Moutsopoulos Niki M
机构信息
Oral Immunity and Inflammation Unit, NIDCR, NIH, Bethesda, MD, USA.
Division of Periodontology, Department of Oral Health and Diagnostic Sciences, UConn Health, Farmington, CT, USA.
出版信息
Bio Protoc. 2017 Dec 20;7(24). doi: 10.21769/BioProtoc.2655.
Abstract
The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene sequencing.
摘要
口腔微生物群被认为是口腔免疫反应的触发因素，特别是在炎症性疾病牙周炎的情况下。本文介绍的方案旨在表征稳态以及免疫或生理扰动期间小鼠模型中的口腔微生物群。在此，我们描述了小鼠口腔微生物群的采样程序、低生物量样本的处理以及随后基于16S rRNA基因测序的微生物群表征。
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