Division of Feeding and Swallowing Rehabilitation, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan.
Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan;
In Vivo. 2022 Sep-Oct;36(5):2116-2125. doi: 10.21873/invivo.12937.
BACKGROUND/AIM: Rapid spread of COVID-19 resulted in the revision of the value of ultraviolet C (UVC) sterilization in working spaces. This study aimed at investigating the UVC sensitivity of eighteen malignant and nonmalignant cell lines, the protective activity of sodium ascorbate against UVC, and whether Dectin-2 is involved in UVC sensitivity.
Various cell lines were exposed to UVC for 3 min, and cell viability was determined using the MTT assay. Anti-UV activity was determined as the ratio of 50% cytotoxic concentration (determined with unirradiated cells) to 50% effective concentration (that restored half of the UV-induced loss of viability). Dectin-2 expression was quantified using flow cytometry.
The use of culture medium rather than phosphate-buffered saline is recommended as irradiation solution, since several cells are easily detached during irradiation in phosphate-buffered saline. Oral squamous cell carcinoma cell lines showed the highest UV sensitivity, followed by neuroblastoma, glioblastoma, leukemia, melanoma, lung carcinoma cells, and normal oral and dermal fibroblasts. Human dermal fibroblasts were more resistant than melanoma cell lines; however, both expressed Dectin-2. Sodium ascorbate at micromolar concentrations eliminated the cytotoxicity of UVC in these cell lines.
Normal cells are generally UVC-resistant compared to corresponding malignant cells, which have higher growth potential. Dectin-2 protein expression itself may not be determinant of UVC sensitivity.
背景/目的：COVID-19 的迅速传播导致工作场所中紫外线 C（UVC）消毒的价值发生了变化。本研究旨在调查 18 种恶性和非恶性细胞系对 UVC 的敏感性、抗坏血酸钠对 UVC 的保护作用，以及 Dectin-2 是否参与 UVC 敏感性。
将各种细胞系暴露于 UVC 中 3 分钟，然后使用 MTT 测定法测定细胞活力。抗紫外线活性被定义为 50%细胞毒性浓度（用未辐照的细胞确定）与 50%有效浓度（恢复 UV 诱导的活力损失的一半）的比值。使用流式细胞术定量测定 Dectin-2 的表达。
建议使用培养基而不是磷酸盐缓冲盐水作为照射溶液，因为在磷酸盐缓冲盐水中照射时，几种细胞很容易脱落。口腔鳞状细胞癌细胞系显示出最高的 UV 敏感性，其次是神经母细胞瘤、胶质母细胞瘤、白血病、黑色素瘤、肺癌细胞和正常口腔和皮肤成纤维细胞。人皮肤成纤维细胞比黑色素瘤细胞系更能抵抗，但两者都表达 Dectin-2。毫摩尔浓度的抗坏血酸钠消除了这些细胞系中 UVC 的细胞毒性。
与具有更高生长潜力的相应恶性细胞相比，正常细胞通常对 UVC 具有抗性。Dectin-2 蛋白表达本身可能不是 UVC 敏感性的决定因素。
