National Agri-Food Biotechnology Institute, Sector 81, Sahibzada Ajit Singh Nagar, Mohali, 140306, India.
Department of Biotechnology, Panjab University, Sector 25, Chandigarh, 160014, India.
ACS Appl Mater Interfaces. 2022 Oct 5;14(39):44084-44097. doi: 10.1021/acsami.2c10844. Epub 2022 Sep 13.
The emergence of multidrug resistant (MDR) microorganisms has led to the development of alternative approaches for providing relief from microbial attacks. The mechano-bactericidal action as a substitute for antimicrobials has become the focus of intensive research. In this work, nanostructure-conjugated hydrogel are explored as a flexible dressing against ()-infected skin wounds. Herein gold nanostars (AuNst) with spike lengths reaching 120 nm are probed for antibacterial action. The bacterial killing of >95% is observed for () and (), while up to 60% for Gram-positive AuNst conjugated hydrogel (AuNst@H) reduced >80% colonies of and . In comparison, around 35.4% reduction of colonies are obtained for . The viability assay confirmed the presence of about 85% of living NIH-3T3 cells when grown with hydrogels. An animal wound model is also developed to assess the efficiency of AuNst@H. A significant reduction in wound size is observed on the 10th day in AuNst@H treated animals with fully formed epidermal layers, hair follicles, new blood vessels, and arrector muscles. These findings suggest that novel dressing materials can be developed with antimicrobial nanotextured surfaces.
多药耐药（MDR）微生物的出现促使人们开发替代方法来缓解微生物的攻击。机械杀菌作用作为抗生素的替代品已成为密集研究的焦点。在这项工作中，我们探索了纳米结构缀合水凝胶作为治疗（）感染皮肤伤口的柔性敷料。在此，我们研究了长度达到 120nm 的金纳米星（AuNst）的抗菌作用。实验结果表明，（）和（）的杀菌率>95%，而革兰氏阳性的 AuNst 缀合水凝胶（AuNst@H）的杀菌率高达 60%，对 和 的菌落数减少>80%。相比之下， 对菌落数的减少约为 35.4%。细胞活力测定证实，当与水凝胶一起生长时，NIH-3T3 细胞的存活率约为 85%。还建立了动物伤口模型来评估 AuNst@H 的效率。在 AuNst@H 治疗的动物中，第 10 天观察到伤口面积明显缩小，表皮层、毛囊、新血管和立毛肌完全形成。这些发现表明，可以开发具有抗菌纳米结构表面的新型敷料材料。
