School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
Institute of Traditional Chinese Medicine, Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan, China.
Biochim Biophys Acta Mol Basis Dis. 2022 Dec 1;1868(12):166539. doi: 10.1016/j.bbadis.2022.166539. Epub 2022 Sep 11.
Muscone is the main active compound of Moschus. In this paper, the cardioprotective effect of Muscone on acute myocardial ischemia (AMI) rats and its potential mechanisms were investigated. AMI rat models were established to evaluate the protective effect and antioxidative function of Muscone on the hearts. Moreover, Western blot analysis was conducted to quantify the phosphorylated PI3K and AKT levels in PI3K/Akt pathway for further investigating the mechanism of Muscone. Results showed that Muscone could markedly lessen the infarct size and myocardial injury, improve cardiac function, inhibit cardiomyocyte apoptosis and down-regulate serum reactive oxygen species level as indicated by the decreased MDA, BNP and c-TnI activities and the increased SOD, GSH-px, CAT activities and the expression of Bax protein. In addition, it was revealed that Muscone notably promoted the phosphorylation of PI3K and AKT. These findings denote that Muscone exerts a protective effect in heart via inhibition of oxidative stress and apoptosis, offering new insights into the treatment of CHD and the clinical application of Muscone.
麝香酮是麝香的主要活性成分。本文研究了麝香酮对急性心肌缺血（AMI）大鼠的心脏保护作用及其潜在机制。建立 AMI 大鼠模型，评估麝香酮对心脏的保护作用和抗氧化功能。此外，还进行了 Western blot 分析，以定量测定 PI3K/Akt 通路中磷酸化 PI3K 和 AKT 的水平，进一步研究麝香酮的作用机制。结果表明，麝香酮可显著减轻梗死面积和心肌损伤，改善心功能，抑制心肌细胞凋亡，并降低血清活性氧水平，表现为 MDA、BNP 和 c-TnI 活性降低，SOD、GSH-px、CAT 活性和 Bax 蛋白表达增加。此外，还发现麝香酮显著促进了 PI3K 和 AKT 的磷酸化。这些发现表明，麝香酮通过抑制氧化应激和细胞凋亡对心脏发挥保护作用，为 CHD 的治疗和麝香酮的临床应用提供了新的思路。
