Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggido 10326, Republic of Korea.
Department of Biological and Environmental Science, Dongguk University, Ilsandong-gu, Goyang-si, Gyonggido 10326, Republic of Korea.
Bioresour Technol. 2022 Nov;363:127926. doi: 10.1016/j.biortech.2022.127926. Epub 2022 Sep 12.
Biocatalysts, including live microbial cells/enzymes, have been considered a predominant and advantageous tool for effectively transforming biomass into biofuels and valued biochemicals. However, high production costs, separation, and reusability limit its practical application. Immobilization of single and multi-enzymes by employing different nano-supports have gained massive attention because of its elevated exterior domain and high enzymatic performance. Application of nanobiocatalyst can overcome the drawbacks mainly, stability and reusability, thus reflecting the importance of biomass-based biorefinery to make it profitable and sustainable. This review provides an in-depth, comprehensive analysis of nanobiocatalysts systems concerning nano supports and biocatalytic performance characteristics. Furthermore, the effects of nanobiocatalyst on waste biomass to biofuel and valued bioproducts in the biorefinery approach and their critical assessment are discussed. Lastly, this review elaborates commercialization and market outlooks of the bioconversion process using nanobiocatalyst, followed by different strategies to overcome the limitations and future research directions on nanobiocatalytic-based industrial bioprocesses.
生物催化剂，包括活微生物细胞/酶，已被认为是将生物质有效转化为生物燃料和有价值的生物化学物质的主要优势工具。然而，高生产成本、分离和可重复使用性限制了其实际应用。通过使用不同的纳米载体固定单酶和多酶已经引起了广泛的关注，因为它具有更高的外域和更高的酶性能。纳米生物催化剂的应用可以克服主要的稳定性和可重复使用性的缺点，从而反映了基于生物质的生物炼制的重要性，使其盈利和可持续。本文深入、全面地分析了纳米载体和生物催化性能特征的纳米生物催化剂体系。此外，还讨论了纳米生物催化剂对生物炼制中废生物质转化为生物燃料和有价值生物制品的影响及其关键评估。最后，本文详细阐述了使用纳米生物催化剂进行生物转化过程的商业化和市场前景，以及克服纳米生物催化工业生物过程限制的不同策略和未来研究方向。
