Saito T
Hokkaido Igaku Zasshi. 1987 Mar;62(2):257-68.
The activity, content and true specific activity of superoxide dismutase (SOD) were determined for human erythrocytes of 105 normal healthy subjects. At the same time the activities of erythrocyte SOD are also determined in patients with lung cancer, lymphoma, leukemia and thyroidal dysfunctions. The mean SOD activity of healthy subjects assayed by the method of inhibition of xanthine autoxidation was 11.0 X 10(3) units/g of hemoglobin (Hb). The mean SOD content of healthy subjects assayed by an immunodiffusion method was 456 micrograms/g of Hb. Both the activity and the content of SOD showed normal distributions, while no significant variations in regard to sex and age were detected. A high positive correlation between the activity and the content of SOD was observed in normal healthy subjects (r = 0.77, p less than 0.001). True specific activity was calculated from the levels of activity and content of SOD. The mean true specific activity of SOD in human erythrocytes was 23.7 units/micrograms of SOD. There was no significant difference in true specific activity among age groups. The activity of erythrocyte SOD was determined in 38 patients with lung cancer (n = 15), malignant lymphoma (n = 11) and acute myeloid leukemia (n = 12). Patients with malignant lymphoma and acute myeloid leukemia showed a significant decrease in enzyme activity (p less than 0.01) while the patients with lung cancer (9 squamous cell carcinomas and 6 small cell carcinomas) showed a normal value of SOD activity. Furthermore, patients with malignant lymphoma and acute myeloid leukemia who were in remission and were not being treated with anticancer drugs also showed a significant decrease in SOD activity. These observations therefore indicate that a low level of erythrocyte SOD activity is related to cancer and that degree of the activities varies with the type of cancer. A total of 18 determinations of erythrocyte SOD activity were made on 16 patients with thyroidal dysfunction. Patients with hyperthyroidism showed a significant increase in SOD activity (p less than 0.01), while patients with hypothyroidism showed the same SOD activity as those of healthy subjects. A significantly high positive correlation was found between erythrocyte SOD activity and the level of thyroxine in serum (r = 0.60, p less than 0.01). The author suggests therefore that erythrocyte SOD activity has a close relationship to the state of the thyroid hormones.
测定了105名正常健康受试者的人红细胞中超氧化物歧化酶（SOD）的活性、含量及真实比活性。同时，也测定了肺癌、淋巴瘤、白血病及甲状腺功能障碍患者红细胞SOD的活性。采用黄嘌呤自氧化抑制法测定的健康受试者SOD平均活性为11.0×10³单位/克血红蛋白（Hb）。采用免疫扩散法测定的健康受试者SOD平均含量为456微克/克Hb。SOD的活性和含量均呈正态分布，且未检测到性别和年龄方面的显著差异。在正常健康受试者中观察到SOD活性与含量之间存在高度正相关（r = 0.77，p＜0.001）。根据SOD的活性和含量水平计算真实比活性。人红细胞中SOD的平均真实比活性为23.7单位/微克SOD。各年龄组之间的真实比活性无显著差异。对38例肺癌（n = 15）、恶性淋巴瘤（n = 11）和急性髓系白血病（n = 12）患者测定了红细胞SOD活性。恶性淋巴瘤和急性髓系白血病患者的酶活性显著降低（p＜0.01），而肺癌患者（9例鳞状细胞癌和6例小细胞癌）的SOD活性值正常。此外，处于缓解期且未接受抗癌药物治疗的恶性淋巴瘤和急性髓系白血病患者的SOD活性也显著降低。因此，这些观察结果表明红细胞SOD活性水平低与癌症有关，且活性程度因癌症类型而异。对16例甲状腺功能障碍患者共进行了18次红细胞SOD活性测定。甲状腺功能亢进患者的SOD活性显著升高（p＜0.01），而甲状腺功能减退患者的SOD活性与健康受试者相同。发现红细胞SOD活性与血清甲状腺素水平之间存在显著的高度正相关（r = 0.60，p＜0.01）。因此，作者认为红细胞SOD活性与甲状腺激素状态密切相关。
