Garrido Eva, Climent Estela, Marcos M Dolores, Sancenón Félix, Rurack Knut, Martínez-Máñez Ramón
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain.
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Av. Monforte de Lemos, 3-5, Pabellón 11, Planta 0 28029 Madrid.
Nanoscale. 2022 Sep 29;14(37):13505-13513. doi: 10.1039/d2nr03325a.
We report herein the design of a strip-based rapid test utilizing bio-inspired hybrid nanomaterials for the and detection of the drug scopolamine (SCP) using a smartphone for readout, allowing SCP identification in diluted saliva down to 40 nM in less than 15 min. For this purpose, we prepared a nanosensor based on mesoporous silica nanoparticles loaded with a fluorescent reporter (rhodamine B) and functionalized with bethanechol, a potent agonist of recombinant human muscarinic acetylcholine receptor M (M-AChR). M-AChR interaction with the anchored bethanechol derivative leads to capping of the pores. The sensing mechanism relies on binding of SCP to M-AChR resulting in pore opening and delivery of the entrapped rhodamine B reporter. Moreover, the material was incorporated into strips for lateral-flow assays coupled to smartphone readout, giving fast response time, good selectivity, and exceptional sensitivity. In an attempt to a mobile analytical test system for law enforcement services, we have also developed a dualplex lateral flow assay for SCP and 3,4-methylenedioxypyrovalerone (MDPV) also known as the so-called "cannibal drug".
我们在此报告一种基于试纸条的快速检测方法的设计，该方法利用受生物启发的混合纳米材料，通过智能手机读取结果，用于检测药物东莨菪碱（SCP），能够在不到15分钟的时间内对稀释唾液中的SCP进行鉴定，检测下限低至40 nM。为此，我们制备了一种纳米传感器，该传感器基于负载有荧光报告分子（罗丹明B）的介孔二氧化硅纳米颗粒，并通过氨甲酰甲胆碱（重组人毒蕈碱型乙酰胆碱受体M（M-AChR）的强效激动剂）进行功能化。M-AChR与锚定的氨甲酰甲胆碱衍生物相互作用导致孔被封闭。传感机制依赖于SCP与M-AChR的结合，从而导致孔打开并释放被困的罗丹明B报告分子。此外，该材料被整合到试纸条中用于侧流分析，并与智能手机读取相结合，具有快速响应时间、良好的选择性和出色的灵敏度。为了开发一种适用于执法服务的移动分析测试系统，我们还开发了一种用于SCP和3,4-亚甲基二氧吡咯戊酮（MDPV，也被称为所谓的“丧尸药”）的双工侧流分析方法。
