Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de Valencia, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain; Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, València, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain; Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, IIS La Fe, Valencia, Spain.
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de Valencia, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain; Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, IIS La Fe, Valencia, Spain.
J Control Release. 2020 Jul 10;323:421-430. doi: 10.1016/j.jconrel.2020.04.048. Epub 2020 May 1.
The characteristics and electromechanical properties of conductive polymers together to their biocompatibility have boosted their application as a suitable tool in regenerative medicine and tissue engineering. However, conducting polymers as drug release materials are far from being ideal. A possibility to overcome this drawback is to combine conducting polymers with on-command delivery particles with inherent high-loading capacity. In this scenario, we report here the preparation of conduction polymers containing gated mesoporous silica nanoparticles (MSN) loaded with a cargo that is delivered on command by electro-chemical stimuli increasing the potential use of conducting polymers as controlled delivery systems. MSNs are loaded with Rhodamine B (Rh B), anchored to the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) doped with poly[(4-styrenesulfonic acid)-co-(maleic acid)], functionalized with a bipyridinium derivative and pores are capped with heparin (P3) by electrostatic interactions. P3 releases the entrapped cargo after the application of -640 mV voltage versus the saturated calomel electrode (SCE). Pore opening in the nanoparticles and dye delivery is ascribed to both (i) the reduction of the grafted bipyridinium derivative and (ii) the polarization of the conducting polymer electrode to negative potentials that induce detachment of positively charged heparin from the surface of the nanoparticles. Biocompatibility and cargo release studies were carried out in HeLa cells cultures.
导电聚合物的特性和机电性能及其生物相容性促使它们在再生医学和组织工程中作为一种合适的工具得到应用。然而，将导电聚合物作为药物释放材料还远非理想。克服这一缺点的一种可能性是将导电聚合物与具有固有高载药能力的指令性药物输送颗粒结合使用。在这种情况下，我们在此报告了载有门控介孔硅纳米颗粒 (MSN) 的导电聚合物的制备，这些 MSN 负载有货物，可通过电化学刺激进行指令性释放，从而增加了导电聚合物作为控制释放系统的潜在用途。MSN 负载有罗丹明 B（Rh B），锚定在聚（3,4-乙二氧基噻吩）（PEDOT）上，该聚合物掺杂有聚[(4-苯乙烯磺酸)-共-(马来酸)]，通过静电相互作用功能化有一个双吡啶衍生物，孔用肝素（P3）封端。在相对于饱和甘汞电极（SCE）施加-640 mV 电压后，P3 释放出被包封的货物。纳米颗粒中的孔打开和染料输送归因于：(i) 接枝的双吡啶衍生物的还原，和 (ii) 导电聚合物电极向负电势极化，这导致带正电荷的肝素从纳米颗粒表面脱离。在 HeLa 细胞培养物中进行了生物相容性和货物释放研究。
