Wilkinson P C, Higgins A
Immunology. 1987 Jul;61(3):311-6.
Human blood lymphocytes acquire locomotor capacity during 24 hr culture with mitogens such as monoclonal anti-CD3 antibodies, phytohaemagglutinin (PHA), or Staphylococcus aureus Cowan strain (SAC). Activation of locomotor capacity by these agents is blocked by the presence of cyclosporin A (greater than or equal to 10 ng per ml), as measured by inhibition of the development of morphological polarization of the cells in culture and inhibition of their capacity to invade collagen gels. The response to OKT3 is inhibited by lower doses of cyclosporin than the responses to SAC or PHA. Phorbol myristate acetate (PMA) induces locomotion of lymphocytes in culture that is not inhibited by cyclosporin. Cyclosporin has no effect on the locomotion of lymphocytes that already possess locomotor capacity. Thus it neither inhibits immediate stimulus-induced polarization of lymphocytes direct from blood, nor reverses polarization of lymphocytes that have acquired motility in culture. These results suggest that cyclosporin prevents events, occurring during the G1 phase of growth, that are necessary for non-motile lymphocytes to acquire locomotor capacity, but has no effect on the locomotor mechanism itself in already motile cells.
人血淋巴细胞在与丝裂原（如单克隆抗 CD3 抗体、植物血凝素（PHA）或金黄色葡萄球菌考恩菌株（SAC））进行 24 小时培养期间获得运动能力。这些试剂对运动能力的激活被环孢素 A（≥10 纳克/毫升）的存在所阻断，这通过抑制培养细胞形态极化的发展及其侵入胶原凝胶的能力来衡量。与对 SAC 或 PHA 的反应相比，较低剂量的环孢素就能抑制对 OKT3 的反应。佛波酯肉豆蔻酸酯（PMA）诱导培养中的淋巴细胞运动，而这种运动不受环孢素的抑制。环孢素对已经具备运动能力的淋巴细胞的运动没有影响。因此，它既不抑制直接从血液中获取的淋巴细胞由即时刺激诱导的极化，也不逆转在培养中已获得运动能力的淋巴细胞的极化。这些结果表明，环孢素阻止了在生长的 G1 期发生的、非运动性淋巴细胞获得运动能力所必需的事件，但对已经具有运动能力的细胞的运动机制本身没有影响。
