Haston W S, Shields J M
J Immunol Methods. 1985 Aug 2;81(2):229-37. doi: 10.1016/0022-1759(85)90208-x.
A method is described which greatly simplifies the screening of compounds which are potentially chemotactic for neutrophil leucocytes. Neutrophils isolated from blood by a standardised procedure are greater than 95% spherical in morphology. Addition of chemotactic factors in isotropic, non-gradient, concentrations induces the spherical shape to become polarized. The degree of polarity depends on the concentration of the factor used, as does the percentage of cells which become polarised. All compounds which induce a good response in assays measuring cell accumulation or orientation in gradients induced a consistent polarizing response in non-gradient conditions with the cells held in suspension. The advantage of this simplified assay over methods currently in use are discussed.
本文描述了一种极大简化对嗜中性白细胞具有潜在趋化性的化合物筛选的方法。通过标准化程序从血液中分离出的嗜中性粒细胞在形态上大于95%呈球形。在各向同性、无梯度浓度下添加趋化因子会使球形变为极化状态。极化程度取决于所用因子的浓度，发生极化的细胞百分比也是如此。在测量细胞在梯度中积累或定向的试验中能引发良好反应的所有化合物，在细胞悬浮于无梯度条件下时，都会引发一致的极化反应。讨论了这种简化试验相对于当前使用方法的优势。
