Chakrabortty Sudipto K, Khodor Yevgenia L, Kitchen Robert R, Miller Dulaney L, Babcock Kailey M, Manning Kyle S, Lang Steven P, Tadigotla Vasisht, Yu Wei, Bershad Eric, Skog Johan, Zanello Susana
Exosome Diagnostics, a Bio-Techne brand, Waltham, MA, USA.
Baylor College of Medicine, Houston, TX, USA.
NPJ Microgravity. 2022 Sep 14;8(1):40. doi: 10.1038/s41526-022-00225-4.
Molecular profiling to characterize the effects of environmental exposures is important from the human health and performance as well as the occupational medicine perspective in space exploration. We have developed a novel exosome-based platform that allows profiling of biological processes in the body from a variety of body fluids. The technology is suitable for diagnostic applications as well as studying the pathophysiology of the Space Associated Neuro-Ocular Syndrome in astronauts and monitoring patients with chronically impaired cerebrospinal fluid drainage or elevated intracranial pressure. In this proof-of-concept, we demonstrate that: (a) exosomes from different biofluids contain a specific population of RNA transcripts; (b) urine collection hardware aboard the ISS is compatible with exosome gene expression technology; (c) cDNA libraries from exosomal RNA can be stored in dry form and at room temperature, representing an interesting option for the creation of longitudinal molecular catalogs that can be stored as a repository for retrospective analysis.
从人类健康与表现以及太空探索中的职业医学角度来看，通过分子谱分析来表征环境暴露的影响非常重要。我们开发了一种基于外泌体的新型平台，该平台能够从多种体液中对体内的生物过程进行谱分析。这项技术适用于诊断应用，也适用于研究宇航员的空间相关神经眼科综合征的病理生理学，以及监测脑脊液引流长期受损或颅内压升高的患者。在这个概念验证中，我们证明：(a) 来自不同生物流体的外泌体包含特定的RNA转录本群体；(b) 国际空间站上的尿液收集硬件与外泌体基因表达技术兼容；(c) 来自外泌体RNA的cDNA文库可以以干燥形式在室温下储存，这为创建纵向分子目录提供了一个有趣的选择，这些目录可以作为回顾性分析的储存库进行保存。
