《GENESTAR 商业太空任务生物样本采集、生物样本库建设及组学数据生成手册》

The GENESTAR manual for biospecimen collection biobanking and omics data generation from commercial space missions.

作者信息

Krishnavajhala Aparna, Gingras Marie-Claude, Urquieta Emmanuel, Chao Hsu, Bandaranaike Dilrukshi, Chen Yi, Bhamidipati Sravya, Korchina Viktoriya, Griffin S Michelle, Masternak Michal M, Moreno Hannah, Mohammed Javid, Murugan Mullai, Posey Jennifer E, Wu Jimmy H, Muzny Donna, Gibbs Richard A, Doddapaneni Harsha

机构信息

Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.

Center for Space Medicine, Baylor College of Medicine, Houston, TX, USA.

出版信息

NPJ Microgravity. 2025 May 14;11(1):16. doi: 10.1038/s41526-025-00472-1.

DOI:10.1038/s41526-025-00472-1

PMID:40368955

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12078511/

Abstract

The surge in commercial and civilian spaceflight enables the systematic and longitudinal, large-scale biospecimen collection to understand the prospective effects of space travel on human health. The Genomics and Space Medicine (Space Omics) project at BCM-HGSC involves a comprehensive biospecimen collection plan from commercial/private space flight participants. The manuscript addresses the critical gaps in the biospecimen collection process including details of the informed consent process, a provision for subjects to obtain custom CLIA-WGS reports, a data dictionary and a LIMS enabled biobank. The manuscript also discusses the biospecimens collection, processing methodologies and nucleic acid suitability for Omics data generation. Results from Axiom-2 mission where, 339 biospecimens were collected using 'Genomic Evaluation of Space Travel and Research (GENESTAR)' manual, at two different sites, showed that 98% of the blood samples and 91.6% of the non-blood samples passed the QC requirements for Omics assays, underscoring the reliability and effectiveness of the GENESTAR manual.

摘要

商业和民用航天的蓬勃发展使得系统、长期、大规模地采集生物样本成为可能，有助于了解太空旅行对人类健康的潜在影响。贝勒医学院人类基因组测序中心的基因组学与太空医学（太空组学）项目涉及一项针对商业/私人太空飞行参与者的全面生物样本采集计划。本文阐述了生物样本采集过程中的关键空白，包括知情同意过程的细节、为受试者提供定制的CLIA-WGS报告的规定、数据字典以及启用LIMS的生物样本库。本文还讨论了生物样本的采集、处理方法以及用于组学数据生成的核酸适用性。在公理-2任务中，在两个不同地点使用“太空旅行与研究基因组评估（GENESTAR）”手册采集了339份生物样本，结果显示98%的血液样本和91.6%的非血液样本通过了组学检测的质量控制要求，这凸显了GENESTAR手册的可靠性和有效性。