Yunnan University of Chinese Medicine, Kunming, 650500, Yunnan Province, China.
Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong Province, China.
BMC Urol. 2022 Sep 14;22(1):152. doi: 10.1186/s12894-022-01037-0.
Kidney renal papillary cell carcinoma (KIRP) is a dangerous cancer, which accounts for 15-20% of all kidney malignancies. Ferroptosis is a rare kind of cell death that overcomes medication resistance. Ferroptosis-related long non-coding RNAs (LNCRNAs) in KIRP, remain unknown.
We wanted to express how ferroptosis-related LNCRNAs interact with immune cell infiltration in KIRP. Gene set enrichment analysis in the GO and KEGG databases were used to explore gene expression enrichment. The prognostic model was constructed using Lasso regression. In addition, we also analyzed the modifications in the tumor microenvironment (TME) and immunological association.
The expression of LNCRNA was closely connected to the ferroptosis, according to co-expression analyses. CASC19, AC090197.1, AC099850.3, AL033397.2, LINC00462, and B3GALT1-AS1 were found to be significantly increased in the high-risk group, indicating that all of these markers implicates the malignancy processes for KIRP patients and may be cancer-promoting variables. LNCTAM34A and AC024022.1 were shown to be significantly elevated in the low-risk group; these might represent as the KIRP tumor suppressor genes. According to the TCGA, CCR, and inflammation-promoting genes were considered to be significantly different between the low-risk and high-risk groups. The expression of CD160, TNFSF4, CD80, BTLA, and TNFRSF9 was different in the two risk groups.
LNCRNAs associated with ferroptosis were linked to the occurrence and progression of KIRP. Ferroptosis-related LNCRNAs and immune cell infiltration in the TME may be potential biomarkers in KIRP that should be further investigated.
肾乳头状细胞癌（KIRP）是一种危险的癌症，占所有肾脏恶性肿瘤的 15-20%。铁死亡是一种罕见的细胞死亡方式，可克服耐药性。KIRP 中与铁死亡相关的长链非编码 RNA（lncRNA）尚不清楚。
我们旨在研究铁死亡相关 lncRNA 如何与 KIRP 中的免疫细胞浸润相互作用。GO 和 KEGG 数据库中的基因集富集分析用于探索基因表达富集。使用 Lasso 回归构建预后模型。此外，我们还分析了肿瘤微环境（TME）和免疫相关性的改变。
根据共表达分析，lncRNA 的表达与铁死亡密切相关。CASC19、AC090197.1、AC099850.3、AL033397.2、LINC00462 和 B3GALT1-AS1 在高危组中显著升高，表明所有这些标志物都暗示了 KIRP 患者的恶性进程，可能是促进癌症的变量。在低危组中，lncRNA LECTM34A 和 AC024022.1 明显升高；这些可能代表 KIRP 的肿瘤抑制基因。根据 TCGA，CCR 和促炎基因在低危组和高危组之间存在显著差异。两个风险组中 CD160、TNFSF4、CD80、BTLA 和 TNFRSF9 的表达不同。
与铁死亡相关的 lncRNA 与 KIRP 的发生和发展有关。TME 中与铁死亡相关的 lncRNA 和免疫细胞浸润可能是 KIRP 的潜在生物标志物，值得进一步研究。
