Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Int J Food Microbiol. 2022 Dec 2;382:109901. doi: 10.1016/j.ijfoodmicro.2022.109901. Epub 2022 Aug 28.
Aquatic organisms are too susceptible to the increased growth of bacterial contamination. It seems that preventive measures should be prioritized to reduce bacterial load, and improve the health situation of marine-based product consumers. Hence, this study is aimed at molecular investigation of the prevalence of Pseudomonas aeruginosa as one of the most food-borne pathogens, antibiotic resistance, and virulence factor encoding gens in lobster samples.
After the collection of aquatic samples from Isfahan and Chabahar city during the summer and autumn seasons, they were cultured, and confirmed by biochemistry tests. Then, they were investigated for antibiotic resistance by the Kirby Bauer method. Then, antibiotic resistance, virulence factor encoding genes, and Multi-Drug Resistance (MDR) patterns were analyzed. Statistical analysis was done by SPSS through chi-square tests.
Bacterial contamination in samples taken from Isfahan city was higher than in Chabahar city despite having a cooler climate on summer days. Antibiotic resistance to piperacillin in fresh shrimp samples taken in summer In Isfahan city was contrary to its usage as a front-line antibiotic agent for Pseudomonas aeruginosa. Lowered MDR pattern in frozen samples, was related to the varied expression of antibiotic resistance, highlighting the importance of regulations for cold chain in storage, transportation, and distribution of marine samples, especially when compared to fresh shrimps.
Food-borne pathogens, antibiotic resistance, and their virulence factors are of clinical and environmental importance. Results of our study indicated a high rate of frequency for Pseudomonas aeruginosa isolated from marine samples, antibiotic resistance, antibiotic resistance encoding genes, virulence factors encoding genes, and MDR. Maintenance of the cold chain, and proper food processing, have indispensable roles in the preservation, and reduction of Pseudomonas aeruginosa frequency in aquatic organisms.
水生生物对细菌污染的增长过于敏感。似乎应该优先采取预防措施来降低细菌负荷，改善海洋产品消费者的健康状况。因此，本研究旨在对从伊斯法罕和恰巴哈尔市夏季和秋季采集的龙虾样本中分离出的铜绿假单胞菌作为食源性致病菌之一的流行情况进行分子调查，包括其抗生素耐药性和毒力因子编码基因。
采集伊斯法罕和恰巴哈尔市夏季和秋季的水生样本后，进行培养，并通过生化试验进行确认。然后，采用 Kirby Bauer 法检测其抗生素耐药性。然后，分析抗生素耐药性、毒力因子编码基因和多药耐药（MDR）模式。采用 SPSS 通过卡方检验进行统计学分析。
尽管夏季伊市的气候较凉爽，但伊市样本中的细菌污染高于恰市。夏季在伊斯法罕市采集的新鲜对虾样本对哌拉西林的抗生素耐药性与将其作为铜绿假单胞菌一线抗生素的用途相悖。冷冻样本中 MDR 模式降低与抗生素耐药性的不同表达有关，突出了冷链在海洋样本储存、运输和分发方面的重要性，特别是与新鲜对虾相比。
食源性致病菌、抗生素耐药性及其毒力因子具有临床和环境重要性。我们的研究结果表明，从海洋样本中分离出的铜绿假单胞菌、抗生素耐药性、抗生素耐药基因、毒力因子编码基因和 MDR 的频率很高。保持冷链和适当的食品加工对水生生物中铜绿假单胞菌的保存和减少具有不可或缺的作用。
