Ikehara M, Fukui T, Kakiuchi N
Nucleic Acids Res. 1978 Jun;5(6):1877-87. doi: 10.1093/nar/5.6.1877.
2'-Deoxy-2'-fluoroadenosine was chemically transformed to its 5'-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2'-deoxy-2'-fluoroadenylic acid) [poly(Af)]. Polymerization proceeded smoothly as in the case of poly(A) and the yield of the polymerization was 55%. The UV absorption spectra of poly(Af) closely resembled those of poly(A) and the hypochromicity was 32% at pH 7.0. The CD profile at 25 degrees and neutrality showed similar pattern to that of other poly(2'-deoxy-2'-halogenoadenylic acids) with somewhat larger [theta] values both in the positive and negative maxima. Acid titration of poly(Af) showed a transition point at pH 5.2 and the Tm of the acid form was 37 degrees which was significantly lower than that of poly(A), but similar to that of poly(2'-azido-2'-deoxyadenylic acid). Poly(Af) formed 1:1 and 1:2 complexes with poly-(U) having Tm of 49 degrees and 62 degrees at 0.04M and 0.15M Na(+) concentration, respectively. Poly(Af) also formed a 1:2 complex with poly(I) and its Tm was 36 degrees at 0.05M Na(+) concentration. These data showed that poly(Af) has rather similar properties to those of poly(A), but not to poly(dA).
2'-脱氧-2'-氟腺苷被化学转化为其5'-二磷酸，并与多核苷酸磷酸化酶聚合，得到聚（2'-脱氧-2'-氟腺苷酸）[聚（Af）]。聚合反应如聚（A）的情况一样顺利进行，聚合产率为55%。聚（Af）的紫外吸收光谱与聚（A）的非常相似，在pH 7.0时的减色效应为32%。25℃和中性条件下的圆二色性图谱显示出与其他聚（2'-脱氧-2'-卤代腺苷酸）相似的模式，在正负最大值处的[θ]值略大。聚（Af）的酸滴定显示在pH 5.2处有一个转变点，酸形式的熔解温度为37℃，明显低于聚（A），但与聚（2'-叠氮基-2'-脱氧腺苷酸）相似。聚（Af）在0.04M和0.15M Na(+)浓度下分别与聚（U）形成1:1和1:2的复合物，其熔解温度分别为49℃和62℃。聚（Af）还与聚（I）形成1:2的复合物，在0.05M Na(+)浓度下其熔解温度为36℃。这些数据表明聚（Af）具有与聚（A）相当相似的性质，但与聚（dA）不同。
