聚（5-甲氧基尿苷酸）的物理性质和编码特性。

Physical and coding properties of poly(5-methoxyuridylic) acid.

作者信息

Hillen W, Gassen H G

出版信息

Biochim Biophys Acta. 1979 Apr 26;562(2):207-13. doi: 10.1016/0005-2787(79)90166-7.

DOI:10.1016/0005-2787(79)90166-7

Abstract

The synthesis of poly(mo5U) requires a high concentration (2.7 mg/ml) of polynucleotide phosphorylase as well as a long reaction time (48 h). The resulting polynucleotide has a chain length of approximately 100 nucleotides. It shows no indication of a stable secondary structure. When poly(mo5U) is mixed with poly(A), a triple-stranded complex poly(A) . 2poly(mo5U) is formed. This complex has a melting temperature of 68.5 +/- 0.5 degrees C at 150 mMNa+ and exhibits a hysteresis loop between melting and reformation of the complex having a delta Tm of 11.5 degrees C. Poly-5-methoxyuridylic acid stimulates the binding of Phe-tRNA to 70-S ribosomes but is inactive in directing poly(Phe) synthesis.

摘要

聚（5-甲氧基尿苷）的合成需要高浓度（2.7毫克/毫升）的多核苷酸磷酸化酶以及较长的反应时间（48小时）。所得的多核苷酸链长度约为100个核苷酸。它没有显示出稳定二级结构的迹象。当聚（5-甲氧基尿苷）与聚（A）混合时，会形成三链复合物聚（A）·2聚（5-甲氧基尿苷）。该复合物在150 mM Na⁺ 时的解链温度为68.5±0.5℃，并且在复合物的解链和重新形成之间表现出滞后环，ΔTm为11.5℃。聚-5-甲氧基尿苷酸刺激苯丙氨酰-tRNA与70-S核糖体的结合，但在指导聚（苯丙氨酸）合成方面没有活性。

相似文献

1

Physical and coding properties of poly(5-methoxyuridylic) acid.聚（5-甲氧基尿苷酸）的物理性质和编码特性。

Biochim Biophys Acta. 1979 Apr 26;562(2):207-13. doi: 10.1016/0005-2787(79)90166-7.

2

Physical and coding properties of poly(5-aminouridylic acid) and of 5-aminouridine-containing trinucleotides.

Biochim Biophys Acta. 1975 Oct 15;407(3):347-56. doi: 10.1016/0005-2787(75)90102-1.

3

Polynucleotides. XLIV. Synthesis and properties of poly (2-azaadenylic acid) and poly(2-azainosinic acid).多核苷酸。XLIV。聚（2-氮杂腺苷酸）和聚（2-氮杂肌苷酸）的合成及性质

Biochim Biophys Acta. 1978 Sep 27;520(2):441-51. doi: 10.1016/0005-2787(78)90241-1.

4

Poly(4-thiouridylic acid) as messenger RNA and its application for photoaffinity labelling of the ribosomal mRNA binding site.聚（4-硫代尿苷酸）作为信使核糖核酸及其在核糖体信使核糖核酸结合位点光亲和标记中的应用。

Eur J Biochem. 1977 Apr 15;74(3):447-56. doi: 10.1111/j.1432-1033.1977.tb11411.x.

5

Influence of 2'-O-alkylation on the structure of single-stranded polynucleotides and the stability of 2'-O-alkylated polynucleotide complexes.2'-O-烷基化对单链多核苷酸结构及2'-O-烷基化多核苷酸复合物稳定性的影响

Biochemistry. 1974 Jun 18;13(13):2762-71. doi: 10.1021/bi00710a016.

6

Polynucleotide duplexes based on poly(7-deazaadenylic acid).

Biochim Biophys Acta. 1975 Jun 2;395(1):56-66. doi: 10.1016/0005-2787(75)90233-6.

7

Poly(2-methylthio-7-deazainosinic acid)--hydrophobic stabilization of polynucleotide secondary structure by the 2-methylthio group.聚（2-甲硫基-7-脱氮肌苷酸）——2-甲硫基对多核苷酸二级结构的疏水稳定作用

Nucleic Acids Res. 1983 Sep 10;11(17):6107-20. doi: 10.1093/nar/11.17.6107.

8

Synthesis and properties of poly (6-methylpurinylic acid), poly (6-methoxypurinylic acid), and poly (6-methylthiopurinylic acid).

Biochim Biophys Acta. 1974 Dec 20;374(3):407-16. doi: 10.1016/0005-2787(74)90262-7.

9

Preparation and properties of poly 2'-O-ethylcytidylic acid.聚2'-O-乙基胞苷酸的制备与性质

Nucleic Acids Res. 1976 Mar;3(3):817-24. doi: 10.1093/nar/3.3.817.

10

Polyribonucleotides containing thiopurines: synthesis and properties of poly (6-thioguanylic acid).含硫代嘌呤的多聚核糖核苷酸：聚（6-硫代鸟苷酸）的合成与性质

Biochemistry. 1976 Oct 5;15(20):4386-9. doi: 10.1021/bi00665a006.

引用本文的文献

1

On the conformation of 5-substituted uridines as studied by proton magnetic resonance.通过质子磁共振研究5-取代尿苷的构象

Nucleic Acids Res. 1983 Feb 25;11(4):1167-80. doi: 10.1093/nar/11.4.1167.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。