聚（5-甲氧基尿苷酸）的物理性质和编码特性。

Physical and coding properties of poly(5-methoxyuridylic) acid.

作者信息

Hillen W, Gassen H G

出版信息

Biochim Biophys Acta. 1979 Apr 26;562(2):207-13. doi: 10.1016/0005-2787(79)90166-7.

Abstract

The synthesis of poly(mo5U) requires a high concentration (2.7 mg/ml) of polynucleotide phosphorylase as well as a long reaction time (48 h). The resulting polynucleotide has a chain length of approximately 100 nucleotides. It shows no indication of a stable secondary structure. When poly(mo5U) is mixed with poly(A), a triple-stranded complex poly(A) . 2poly(mo5U) is formed. This complex has a melting temperature of 68.5 +/- 0.5 degrees C at 150 mMNa+ and exhibits a hysteresis loop between melting and reformation of the complex having a delta Tm of 11.5 degrees C. Poly-5-methoxyuridylic acid stimulates the binding of Phe-tRNA to 70-S ribosomes but is inactive in directing poly(Phe) synthesis.

摘要

聚（5-甲氧基尿苷）的合成需要高浓度（2.7毫克/毫升）的多核苷酸磷酸化酶以及较长的反应时间（48小时）。所得的多核苷酸链长度约为100个核苷酸。它没有显示出稳定二级结构的迹象。当聚（5-甲氧基尿苷）与聚（A）混合时，会形成三链复合物聚（A）·2聚（5-甲氧基尿苷）。该复合物在150 mM Na⁺ 时的解链温度为68.5±0.5℃，并且在复合物的解链和重新形成之间表现出滞后环，ΔTm为11.5℃。聚-5-甲氧基尿苷酸刺激苯丙氨酰-tRNA与70-S核糖体的结合，但在指导聚（苯丙氨酸）合成方面没有活性。