School of Pharmaceutical Engineering, Xinyang Agriculture and Forestry University, Xingyang 464000, Henan, China.
Contrast Media Mol Imaging. 2022 Aug 30;2022:7701198. doi: 10.1155/2022/7701198. eCollection 2022.
Research on the active components of medicinal plants has always been the focus of research, and research on the active components of medicinal plant endophytic fungi and their secondary metabolites has also attracted widespread attention. Endophytic fungi of medicinal plants are widely distributed and are ubiquitous in various biological groups in nature. Rehmannia glutinosa contains a variety of active ingredients, which are regarded as the top grade of Chinese medicinal materials. It is of certain significance to study endophytic fungi and their metabolites of Rehmannia glutinosa. In this paper, endophytic fungi and their secondary metabolites of Rehmannia glutinosa were studied using fingerprint technology, which initially understands the diversity of endophytic fungi in Rehmannia glutinosa. In this paper, the roots and leaves of Rehmannia glutinosa were used as experimental materials. The fungi were cultured in the medium, the fungi were isolated and purified by the tissue block method, the fungal growth of Rehmannia glutinosa in different parts was determined, and the types of endophytic fungi were identified by microscopic identification and fingerprinting. The isolated strains were tested for biological activity using oryza oryzae spores, and highly active strains were screened. Fermentation products of endophytic fungi were separated and purified by chromatography, and the structure of the compounds was identified by nuclear magnetic resonance spectroscopy. Through the above studies, the population structure of endophytic fungi of Rehmannia glutinosa was determined, 3 highly active strains were found, and the structures of 7 endophytic fungi metabolites were identified, of which 3 were newly discovered compounds.
药用植物活性成分的研究一直是研究的重点，而药用植物内生真菌及其次生代谢产物的研究也引起了广泛关注。药用植物内生真菌分布广泛，存在于自然界各种生物群中。地黄含有多种活性成分，被视为中药材中的上品。研究地黄内生真菌及其代谢产物具有一定的意义。本文采用指纹技术对地黄内生真菌及其次生代谢产物进行了研究，初步了解了地黄内生真菌的多样性。本文以地黄的根和叶为实验材料，在培养基中培养真菌，采用组织块法分离和纯化真菌，测定地黄不同部位内生真菌的生长情况，通过显微镜鉴定和指纹鉴定鉴定内生真菌的种类。采用稻瘟霉孢子对分离菌株进行生物活性测试，筛选高活性菌株。采用色谱法对内生真菌发酵产物进行分离和纯化，通过核磁共振波谱法鉴定化合物结构。通过以上研究，确定了地黄内生真菌的种群结构，发现了 3 株高活性菌株，鉴定了 7 种内生真菌代谢产物的结构，其中 3 种为新发现的化合物。
