Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China.
Int J Biol Macromol. 2020 Oct 15;161:810-817. doi: 10.1016/j.ijbiomac.2020.06.124. Epub 2020 Jun 15.
Phragmites rhizoma (PR) is comprised of polysaccharides as its main active component. Currently, there are few studies on polysaccharides from PR, especially in purification and structure. In this study, an acidic polysaccharide (PRP-2) was obtained from PR by ultrasonic assisted extraction and secondary column chromatography purification (Diethylaminoethyl cellulose-52 (DEAE-52) and Sephadex G-100). Its structural characteristics were investigated by the gel permeation chromatography (GPC), gas chromatography and mass spectrometry (GC-MS), infrared spectroscopy (IR) and nuclear magnetic resonance (NMR) spectroscopy. The results showed that PRP-2 possessed the molecular weight of 20,332 Da and contained total sugars (71.73%), uronic acids (7.51%), proteins (0.57%) and sulfate radical (9.38%). The polysaccharide was composed of Galactose (34.70%), Fucose (36.15%) and a small amount of Rhamnose (0.88%) with a molar ratio of 39.50: 41.15: 1.00. It consisted of three sugar residues, →3)-β-D-GalpA-(1→, →2, 3)-α-L-Fucp-(1→ and α-L-Fucp (4SO) -(1→. PRP-2 could protect RAW246.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), inhibit the LPS-induced NO production in RAW246.7 macrophages, which displayed its anti-inflammatory activity. Therefore, PRP-2 has the potential to be used as a functional component.
芦根（Phragmites rhizoma，PR）的主要活性成分为多糖。目前，关于 PR 多糖的研究较少，尤其是在纯化和结构方面。本研究采用超声辅助提取和二次柱层析纯化（二乙氨基乙基纤维素-52（DEAE-52）和葡聚糖凝胶 G-100）从 PR 中得到一种酸性多糖（PRP-2）。采用凝胶渗透色谱（GPC）、气相色谱和质谱（GC-MS）、红外光谱（IR）和核磁共振（NMR）光谱对其结构特征进行了研究。结果表明，PRP-2 的分子量为 20332 Da，总糖含量（71.73%）、糖醛酸含量（7.51%）、蛋白质含量（0.57%）和硫酸根含量（9.38%）。多糖由半乳糖（34.70%）、岩藻糖（36.15%）和少量鼠李糖（0.88%）组成，摩尔比为 39.50:41.15:1.00。它由三个糖残基组成，→3）-β-D-GalpA-（1→，→2, 3）-α-L-Fucp-（1→和α-L-Fucp（4SO）-（1→。PRP-2 能保护 RAW246.7 巨噬细胞免受脂多糖（LPS）诱导的细胞毒性作用，抑制 LPS 诱导的 RAW246.7 巨噬细胞中 NO 的产生，显示其抗炎活性。因此，PRP-2 具有作为功能成分的潜力。
