Department of Medical Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
J Clin Lab Anal. 2013 Jul;27(4):312-6. doi: 10.1002/jcla.21604.
Recent technical developments have focused on the full automation of urinalyses, however the manual microscopic analysis of urine sediment is considered the reference method. The aim of this study was to compare the performances of the LabUMat-UriSed and the H800-FUS100 with manual microscopy, and with each other.
The urine sediments of 332 urine samples were examined by these two devices (LabUMat-UriSed, H800-FUS100) and manual microscopy.
The reproducibility of the analyzers, UriSed and Fus100 (4.1-28.5% and 4.7-21.2%, respectively), was better than that with manual microscopy (8.5-33.3%). The UriSed was more sensitive for leukocytes (82%), while the Fus-100 was more sensitive for erythrocyte cell counting (73%). There were moderate correlations between manual microscopy and the two devices, UriSed and Fus100, for erythrocyte (r = 0.496 and 0.498, respectively) and leukocyte (r = 0.597 and 0.599, respectively) cell counting however the correlation between the two devices was much better for erythrocyte (r = 0.643) and for leukocyte (r = 0.767) cell counting.
It can be concluded that these two devices showed similar performances. They were time-saving and standardized techniques, especially for reducing preanalytical errors such as the study time, centrifugation, and specimen volume for sedimentary analysis; however, the automated systems are still inadequate for classifying the cells that are present in pathological urine specimens.
最近的技术发展集中在尿液分析的完全自动化上，然而尿液沉渣的手动显微镜分析仍被认为是参考方法。本研究旨在比较 LabUMat-UriSed 和 H800-FUS100 与手动显微镜以及彼此之间的性能。
使用这两种设备（LabUMat-UriSed、H800-FUS100）和手动显微镜检查 332 个尿液样本的尿液沉淀物。
分析仪 UriSed 和 Fus100 的重现性（分别为 4.1-28.5%和 4.7-21.2%）优于手动显微镜（8.5-33.3%）。UriSed 对白细胞更敏感（82%），而 Fus-100 对红细胞计数更敏感（73%）。手动显微镜与两种设备 UriSed 和 Fus100 之间的红细胞（r = 0.496 和 0.498，分别）和白细胞（r = 0.597 和 0.599，分别）计数均有中度相关性，但两种设备之间的相关性更好红细胞（r = 0.643）和白细胞（r = 0.767）细胞计数。
可以得出结论，这两种设备表现出相似的性能。它们是节省时间和标准化的技术，特别是可以减少分析沉淀物所需的预分析错误，例如研究时间、离心和标本量；然而，自动化系统仍然不足以对存在于病理性尿液标本中的细胞进行分类。
