Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College (Autonomous), Chennai, Tamilnadu 600106, India.
Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College (Autonomous), Chennai, Tamilnadu 600106, India.
Microbiol Res. 2022 Dec;265:127184. doi: 10.1016/j.micres.2022.127184. Epub 2022 Sep 6.
Hydrocarbon contamination is continuing to be a serious environmental problem because of their toxicity. Hydrocarbon components have been known to be carcinogens and neurotoxic organic pollutants. The physical and chemical methods of petroleum removal have become ineffective and also are very costly. Therefore, bioremediation is considered the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization.The current study also concentrates on bioremediation of petroleum products by bacterium isolated from petroleum hydrocarbon contaminated soil. The current work shows that bacterial strains obtained from a petroleum hydrocarbon contaminated environment may degrade petroleum compounds. Two strains Bacillus licheniformis ARMP2 and Pseudomonas aeruginosa ARMP8 were identified as petroleum-degrading bacteria of the isolated bacterial colonies. The best growth conditions for the ARMP2 strain were determined to be pH 9, temperature 29 °C with sodium nitrate as its nitrogen source, whereas for the ARMP8 strain the optimal growth was found at pH 7, temperature 39 °C, and ammonium chloride as the nitrogen source. Both strains were shown to be effective at degrading petroleum chemicals confirmed by GCMS. Overall petroleum product degradation efficiency of the strains ARMP2 and ARMP8 was about 88 % and 73 % respectively in 48 h.The strains Bacillus licheniformis ARMP2 and Pseudomonas aeruginosa ARMP8 were shown to be effective at degrading petroleum compounds in the current study. Even greater results might be obtained if the organisms were utilised in consortia or the degradation time period was extended.
烃类污染因其毒性仍在继续成为一个严重的环境问题。烃类成分已被证明是致癌物质和神经毒性有机污染物。去除石油的物理和化学方法已经变得无效，而且成本也非常高。因此，生物修复被认为是处理这些污染场地的有前途的技术，因为它具有成本效益，并将导致完全矿化。目前的研究还集中在从石油烃污染土壤中分离出的细菌对石油产品的生物修复上。目前的工作表明，从石油烃污染环境中获得的细菌菌株可能会降解石油化合物。从分离的细菌菌落中鉴定出两种菌株为石油降解菌：地衣芽孢杆菌 ARMP2 和铜绿假单胞菌 ARMP8。确定 ARMP2 菌株的最佳生长条件为 pH9、温度 29°C，硝酸钠为氮源，而 ARMP8 菌株的最佳生长条件为 pH7、温度 39°C，氯化铵为氮源。通过 GCMS 证实，这两种菌株都能有效地降解石油化学品。在 48 小时内，菌株 ARMP2 和 ARMP8 对石油产品的降解效率分别约为 88%和 73%。在本研究中，地衣芽孢杆菌 ARMP2 和铜绿假单胞菌 ARMP8 被证明能有效地降解石油化合物。如果将这些生物用于联合体或延长降解时间，可能会获得更大的结果。
