Zhang Xiaolei, Yang Meitao, Lv Dan, Xie Yin, Sun Yanan, Zhang Yanling, He Mengzhou, Liu Haiyi, Li Fanfan, Deng Dongrui
Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095, Jiefang Ave, Wuhan, Hubei, China.
Department of Gynecology and Obstetrics, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
Open Life Sci. 2022 Sep 3;17(1):1043-1052. doi: 10.1515/biol-2022-0462. eCollection 2022.
The Ca-activated potassium (KCa) channels are involved in many cellular functions, but their roles in trophoblasts are unclear. This study aimed to clarify the effects of KCa channels on the biological behavior of trophoblasts. The localization and expression of the three types of KCa channels, including large-conductance KCa channels (BKCa), intermediate-conductance KCa channels (IKCa), and small-conductance KCa channels (SKCa), were detected in human chorionic villi taken from pregnant women between 5 and 8 weeks of gestation ( = 15) and HTR-8/SVneo cells. The effects of KCa channels on proliferation, apoptosis, and migration of HTR-8/SVneo cells were examined by using the activators or inhibitors of KCa channels. Results showed that KCa channels were mainly localized on the membrane and in the cytoplasm of trophoblasts in human chorionic villi and HTR-8/SVneo cells. The proliferation and migration of HTR-8/SVneo cells were inhibited by activating KCa channels. Apoptosis of trophoblasts was promoted through activating BKCa channels but was not affected by neither activating nor inhibiting IKCa and SKCa channels. This study substantiated the abovementioned biological roles of KCa channels in trophoblast cells, which is fundamental to further research on whether dysfunction of KCa channels is involved in the pathogenesis of pregnancy-related complications.
钙激活钾（KCa）通道参与多种细胞功能，但其在滋养层细胞中的作用尚不清楚。本研究旨在阐明KCa通道对滋养层细胞生物学行为的影响。在取自妊娠5至8周孕妇（n = 15）的人绒毛膜绒毛和HTR-8/SVneo细胞中检测了三种类型KCa通道的定位和表达，包括大电导KCa通道（BKCa）、中等电导KCa通道（IKCa）和小电导KCa通道（SKCa）。通过使用KCa通道激活剂或抑制剂，研究了KCa通道对HTR-8/SVneo细胞增殖、凋亡和迁移的影响。结果表明，KCa通道主要定位于人绒毛膜绒毛和HTR-8/SVneo细胞中滋养层细胞的细胞膜和细胞质。激活KCa通道可抑制HTR-8/SVneo细胞的增殖和迁移。激活BKCa通道可促进滋养层细胞凋亡，但激活或抑制IKCa和SKCa通道对其无影响。本研究证实了KCa通道在滋养层细胞中的上述生物学作用，这对于进一步研究KCa通道功能障碍是否参与妊娠相关并发症的发病机制至关重要。
