Xu Jing, Wei Xue-Ping, Liu Jiu-Shi, Qi Yao-Dong, Zhang Ben-Gang, Liu Hai-Tao, Xiao Pei-Gen
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
Engineering Research Center of Tradition Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
Chin Herb Med. 2021 May 28;13(3):416-420. doi: 10.1016/j.chmed.2021.05.002. eCollection 2021 Jul.
Dianjixueteng is a geoherb in Yunnan Province, the source plant of which is . However, the formation of this geoherb is not clear in genetic mechanism, in which genome size is the first step that should be known on the genomic level. In this study we aimed to estimate the genome sizes of source plants of and three related herbs , , and . by flow cytometry (FCM) and make a comparison.
The genome sizes of , , and . , i.e., the source plants of Dianjixueteng and its relative medicinal materials, were estimated by FCM. The nuclei of were isolated using modified LB01 buffer, for the rest species, by the Galbraith's buffer.
The genome sizes of , , , and were 7.36, 7.12, 7.01, and 5.15 pg/1C, respectively. Genome size of had no significant variation with those of and ( = 0.296), which was significantly larger than that of . .
Genome size can not distinguish from and , but could distinguish them from , which lays the foundation for future studies on genetic mechanism of the geoherb formation.
滇鸡血藤是云南省的道地药材，其来源植物为 。然而，这种道地药材形成的遗传机制尚不清楚，其中基因组大小是在基因组水平上首先需要了解的。在本研究中，我们旨在通过流式细胞术（FCM）估计 及其三种相关药材 、 和 的来源植物的基因组大小并进行比较。
采用FCM估计滇鸡血藤及其相关药材的来源植物 、 、 和 的基因组大小。 的细胞核采用改良的LB01缓冲液分离，其余物种采用Galbraith缓冲液分离。
、 、 和 的基因组大小分别为7.36、7.12、7.01和5.15 pg/1C。 的基因组大小与 和 的基因组大小无显著差异（ = 0.296），但显著大于 。
基因组大小不能区分 和 ，但能将它们与 区分开来，这为今后研究道地药材形成的遗传机制奠定了基础。
