Zhang Nan, Tian Yuan, Wang Yu, Fan Yuling, Zhang Yue, Xing Xinyue, Nan Bo, Ai Zhiyi, Li Xia, Wang Yuhua
College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, 130118, China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, 130118, China.
Food Chem Toxicol. 2022 Nov;169:113433. doi: 10.1016/j.fct.2022.113433. Epub 2022 Sep 16.
Cyclophosphamide (CTX) is a widely used anticancer drug that can cause liver injury, but there is no effective treatment available at present. The antioxidant properties of Lactobacillus plantarum Lp2 in vitro and its effect on CTX-induced liver injury in mice were investigated thoroughly. The order of antioxidant capacity of the fermentate of Lp2 was as followed: fermented supernatant > cell-free extract > intact cell. BALB/c mice were intraperitoneally injected with 80 mg/kg BW/d CTX for 3 days to build a liver injury model, then treated with Lp2 fermented supernatant (Lp2-s) and Lp2 culture broth (Lp2). After 10 days, the indicators of oxidative stress and liver injury were measured. Both Lp2-s and Lp2 restored the levels of T-SOD, CAT, GSH-Px, MDA, GSH, ALT, and AST. The western blotting results showed that Lp2-s and Lp2 ameliorated CTX-induced oxidative damage and hepatocyte apoptosis via inhibiting MAPKs pathway and strengthening Nrf2/HO-1/NQO1 antioxidant defense system, thus inhibiting the mitochondrial-mediated apoptosis pathway. Therefore, both Lp2-s and Lp2 had similar protective effects on CTX-induced liver injury.
环磷酰胺（CTX）是一种广泛使用的抗癌药物，可导致肝损伤，但目前尚无有效的治疗方法。深入研究了植物乳杆菌Lp2的体外抗氧化特性及其对CTX诱导的小鼠肝损伤的影响。Lp2发酵产物的抗氧化能力顺序如下：发酵上清液＞无细胞提取物＞完整细胞。将BALB/c小鼠腹腔注射80mg/kg体重/天的CTX，连续3天以建立肝损伤模型，然后用Lp2发酵上清液（Lp2-s）和Lp2培养液（Lp2）进行处理。10天后，检测氧化应激和肝损伤指标。Lp2-s和Lp2均恢复了T-SOD、CAT、GSH-Px、MDA、GSH、ALT和AST的水平。蛋白质印迹结果表明，Lp2-s和Lp2通过抑制MAPKs通路和增强Nrf2/HO-1/NQO1抗氧化防御系统，改善CTX诱导的氧化损伤和肝细胞凋亡，从而抑制线粒体介导的凋亡途径。因此，Lp2-s和Lp2对CTX诱导的肝损伤具有相似的保护作用。
